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Literature DB >> 16345417

Continuous Cultivation for Apparent Optimization of Defined Media for Cellulomonas sp. and Bacillus cereus.

R J Summers¹, D P Boudreaux, V R Srinivasan.

Abstract

Steady-state continuous culture was used to optimize lean chemically defined media for a Cellulomonas sp. and Bacillus cereus strain T. Both organisms were extremely sensitive to variations in trace-metal concentrations. However, medium optimization by this technique proved rapid, and multifactor screening was easily conducted by using a minimum of instrumentation. The optimized media supported critical dilution rates of 0.571 and 0.467 h for Cellulomonas and Bacillus, respectively. These values approximated maximum growth rate values observed in batch culture.

Entities: Chemical Species

Year: 1979 PMID： 16345417 PMCID： PMC243436 DOI： 10.1128/aem.38.1.66-71.1979

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

11 in total

Continuous Cultivation for Apparent Optimization of Defined Media for Cellulomonas sp. and Bacillus cereus.

1. Application of modified Rosenbrock's method for optimization of nutrient media used in microorganism culturing.

2. Growth dynamics of Agrobacterium tumefaciens in chemostat cultures limited by carbon source and mineral nutrients.

3. Effects of iron deficiency and of zinc deficiency on the composition of Mycobacterium smegmatis.

4. Inorganic micronutrient requirements of chlorella. II. Quantitative requirements for iron, manganese, and zinc.

5. Use of response surface methodology for the rapid optimization of microbiological media.

6. Continuous culture used for media optimization.

7. Magnesium-limited growth of Bacillus subtilis, in pure and mixed cultures, in a chemostat.

8. Zinc effects on growth and cynodontin production of helminthosporium cynodontis.

9. Effect of manganese and zinc ions on the growth of Rhizobium.

10. Phosphorus metabolism of Mycobacteria: determination of phosphorus compounds in some Mycobacteria.

1. Development of a minimal medium for Clostridium perfringens by using an anaerobic chemostat.

2. Hexavalent chromium-resistant bacteria isolated from river sediments.

3. Cross-Feeding of a Toxic Metabolite in a Synthetic Lignocellulose-Degrading Microbial Community.