Literature DB >> 16341781

The sensitive determination of nucleic acids using fluorescence enhancement of Eu3+-benzoylacetone-cetyltrimethylammonium bromide-nucleic acid system.

Xia Wu1, Changying Guo, Jinghe Yang, Minqin Wang, Yanjing Chen, Jian Liu.   

Abstract

A new quantitative method for micro amounts of nucleic acids in aqueous solution is proposed using Eu3+-benzoylacetone (BA) complex as fluorescent probe in the presence of cetyltrimethyl-ammonium bromide (CTMAB). Under the optimum condition, the ratio of the fluorescence intensities with and without nucleic acids is proportional to the concentration of nucleic acid in the range of 1.0x10(-9) to 5.0x10(-6) g/mL for herring sperm DNA (hsDNA), 3.0x10(-9) to 1.0x10(-6) g/mL for calf thymus DNA(ctDNA) and 8.0x10(-9) to 1.0x10(-6) g/mL for yeast RNA (yRNA), and their detection limits are 0.33, 0.21 and 0.99 ng/mL, respectively. Actual sample (DNA of Arabidopsis thaliana) was determined satisfactorily. In addition, the interaction mechanism is also investigated.

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Year:  2005        PMID： 16341781     DOI： 10.1007/s10895-005-2971-y

Source DB:  PubMed          Journal:  J Fluoresc        ISSN： 1053-0509            Impact factor:   2.217


  9 in total

1.  Fluorescence characteristics of purines, pyrimidines, and their derivatives: measurement of guanine in nucleic acid hydrolyzates.

Authors:  S UDENFRIEND; P ZALTZMAN
Journal:  Anal Biochem       Date:  1962-01       Impact factor: 3.365

2.  Fluorescence energy transfer between Acridine Orange and Safranine T and its application in the determination of DNA.

Authors:  Y Cao; X He; Z Gao; L Peng
Journal:  Talanta       Date:  1999-06-14       Impact factor: 6.057

3.  Study on a fluorescence-enhanced system of 1,2,4-trihydroxyanthroquinone-Be(2+)-DNA.

Authors:  J Yang; C Lin; G Zhang; Z Gao; X Wu; X Huang
Journal:  Spectrochim Acta A Mol Biomol Spectrosc       Date:  1998-10       Impact factor: 4.098

4.  Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity.

Authors:  H S Rye; J M Dabora; M A Quesada; R A Mathies; A N Glazer
Journal:  Anal Biochem       Date:  1993-01       Impact factor: 3.365

5.  Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids.

Authors:  J Markovits; B P Roques; J B Le Pecq
Journal:  Anal Biochem       Date:  1979-04-15       Impact factor: 3.365

6.  A new fluorometric method for RNA and DNA determination.

Authors:  J B Le Pecq; C Paoletti
Journal:  Anal Biochem       Date:  1966-10       Impact factor: 3.365

7.  New luminescent terbium complex for the determination of DNA.

Authors:  Alla Yegorova; Alexander Karasyov; Axel Duerkop; Igor Ukrainets; Valery Antonovich
Journal:  Spectrochim Acta A Mol Biomol Spectrosc       Date:  2005-01-01       Impact factor: 4.098

8.  Determination of nucleic acids based on shifting the association equilibrium between tetrasulfonated aluminium phthalocyanine and acridine orange.

Authors:  D H Li; X L Chen; Y Fang; J G Xu
Journal:  Analyst       Date:  2001-04       Impact factor: 4.616

9.  Study on the interaction between nucleic acids and cationic surfactants.

Authors:  Rutao Liu; Jinghe Yang; Changxia Sun; Xia Wu; Lei Li; Benyu Su
Journal:  Colloids Surf B Biointerfaces       Date:  2004-03-01       Impact factor: 5.268
  9 in total
  1 in total

1.  Synthesis of novel Eu(III) luminescent probe based on 9- acridinecarboxylic acid skelton for sensing of ds-DNA.

Authors:  Hassan A Azab; Belal H M Hussein; Abdullah I El-Falouji
Journal:  J Fluoresc       Date:  2011-11-09       Impact factor: 2.217
  1 in total

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