OBJECTIVE: To develop and test an adequate method for the study of sperm chemotaxis. DESIGN: A model based on a double-chamber design and a matrix of 0.5% hyaluronic acid was used to test the response of human sperm to chemotactic signals from follicular fluid (FF). RESULTS: A plateau of migration was reached at 10 minutes of incubation; under this condition, 70% of sperm migrated toward chambers with FF, less than 20% to the opposite side, and the rest remained at the site of application. Intra-assay and interassay coefficients of variation using pools of FFs were 17.2% and 23%, respectively. CONCLUSION: These data confirm previous findings and support the use of this novel model for the study of chemotaxis on human gametes.
OBJECTIVE: To develop and test an adequate method for the study of sperm chemotaxis. DESIGN: A model based on a double-chamber design and a matrix of 0.5% hyaluronic acid was used to test the response of human sperm to chemotactic signals from follicular fluid (FF). RESULTS: A plateau of migration was reached at 10 minutes of incubation; under this condition, 70% of sperm migrated toward chambers with FF, less than 20% to the opposite side, and the rest remained at the site of application. Intra-assay and interassay coefficients of variation using pools of FFs were 17.2% and 23%, respectively. CONCLUSION: These data confirm previous findings and support the use of this novel model for the study of chemotaxis on human gametes.
Authors: J H Olson; X Xiang; T Ziegert; A Kittelson; A Rawls; A L Bieber; D E Chandler Journal: Proc Natl Acad Sci U S A Date: 2001-09-18 Impact factor: 11.205