| Literature DB >> 16338594 |
Tomoyuki Nakagawa1, Ryoko Ikehata, Masataka Uchino, Tatsuro Miyaji, Katsumi Takano, Noboru Tomizuka.
Abstract
In the present study, psychrophilic yeasts, which grow on lactose as a sole carbon source at low temperature and under acidic conditions, were isolated from soil from Hokkaido, Japan. The phenotypes and sequences of 28S rDNA of the isolated strains indicated a taxonomic affiliation to Guehomyces pullulans. The isolated strains were able to grow on lactose at below 5 degrees C, and showed cold-active acid beta-galactosidase activity even at 0 degrees C and pH 4.0 in the extracellular fractions. Moreover, K(m) of beta-galactosidase activity for lactose in the extracellular fraction from strain R1 was found to be 50.5 mM at 10 degrees C, and the activity could hydrolyze lactose in milk at 10 degrees C. The findings in this study indicate the possibility that the isolated strains produce novel acid beta-galactosidases that are able to hydrolyze lactose at low temperature.Entities:
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Year: 2005 PMID： 16338594 DOI： 10.1016/j.micres.2005.07.003
Source DB: PubMed Journal: Microbiol Res ISSN： 0944-5013 Impact factor: 5.415