| Literature DB >> 16336960 |
Takafumi Hirao1, Daisuke Nanba, Motonari Tanaka, Hiroshi Ishiguro, Yumi Kinugasa, Yuichiro Doki, Masahiko Yano, Nariaki Matsuura, Morito Monden, Shigeki Higashiyama.
Abstract
Growth factor-mediated stimulation of epithelial cells induces the disassembly of E-cadherin-mediated cell-cell adhesion. We found that overexpression of a disintegrin and metalloprotease 9 (ADAM9) enhanced growth factor-mediated induction of endocytosis and dynamic recycling of E-cadherin in HT29 human colon cancer cells. In addition, ubiquitination and degradation of E-cadherin were reduced in these cells. ADAM9 constitutively interacted with E-cadherin, and the two proteins co-localized at the plasma membrane of HT29 cells. Administration of a metalloprotease inhibitor or overexpression of an ADAM9 mutant lacking metalloprotease activity attenuated growth factor-dependent endocytosis and recycling of E-cadherin as well as scattering of HT29 cells. These results suggest that the metalloprotease activity of ADAM9 mediates growth factor-induced endocytosis and dynamic recycling of E-cadherin and prevents E-cadherin degradation.Entities:
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Year: 2005 PMID： 16336960 DOI： 10.1016/j.yexcr.2005.10.032
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905