OBJECTIVE: To investigate the effects of controlled release microspheres (Ms) incorporating bFGF on the cultured Schwann cells. METHODS: The secondary cultured Schwann cells were divided into three groups according to the different ingredients being added to the DMEM culture medium: bFGF group, and bFGF-PLGA-Ms group, and bFGF-PELA-Ms group. At different times after culture, the proliferative Schwann cells were collected from three groups individually. Then the number of Schwann cells was measured with cell counting method, the viability of Schwann cells was measured with MTT method and the cell cycle of Schwann cells was measured with flow cytometry. RESULTS: The in vitro cellular study showed that 1, 2 days after plate culture, the number of cells and the cell viability of bFGF group were significantly larger than those of bFGF-PLGA-Ms group and bFGF-PELA-Ms group. 3, 4 days after plate culture, the number of cells and the cell viability of bFGF group and bFGF-PLGA-Ms group were significantly larger than those of bFGF-PELA-Ms group. 6, 8 days after plate culture, the number of cells and the cell viability of bFGF-PLGA-Ms group was significantly larger than those of bFGF group and bFGF-PELA-Ms group. For the flow cytometry examination, 2 days after plate culture, the G2/ M+S percentage of bFGF group was the highest, and 4, 8 days after plate culture, the G2/M+S percentage of bFGF-PLGA-Ms group was the highest. CONCLUSION: Free bFGF can promote the proliferation of Schwann cells in a short period, while bFGF-PLGA-Ms can promote the proliferation of Schwann cells in a long period because of the controlled release of bFGF from microspheres. bFGF-PELA-Ms meets the property requirement of controlled release, but the biological activity of released bFGF is destroyed partially.
OBJECTIVE: To investigate the effects of controlled release microspheres (Ms) incorporating bFGF on the cultured Schwann cells. METHODS: The secondary cultured Schwann cells were divided into three groups according to the different ingredients being added to the DMEM culture medium: bFGF group, and bFGF-PLGA-Ms group, and bFGF-PELA-Ms group. At different times after culture, the proliferative Schwann cells were collected from three groups individually. Then the number of Schwann cells was measured with cell counting method, the viability of Schwann cells was measured with MTT method and the cell cycle of Schwann cells was measured with flow cytometry. RESULTS: The in vitro cellular study showed that 1, 2 days after plate culture, the number of cells and the cell viability of bFGF group were significantly larger than those of bFGF-PLGA-Ms group and bFGF-PELA-Ms group. 3, 4 days after plate culture, the number of cells and the cell viability of bFGF group and bFGF-PLGA-Ms group were significantly larger than those of bFGF-PELA-Ms group. 6, 8 days after plate culture, the number of cells and the cell viability of bFGF-PLGA-Ms group was significantly larger than those of bFGF group and bFGF-PELA-Ms group. For the flow cytometry examination, 2 days after plate culture, the G2/ M+S percentage of bFGF group was the highest, and 4, 8 days after plate culture, the G2/M+S percentage of bFGF-PLGA-Ms group was the highest. CONCLUSION: Free bFGF can promote the proliferation of Schwann cells in a short period, while bFGF-PLGA-Ms can promote the proliferation of Schwann cells in a long period because of the controlled release of bFGF from microspheres. bFGF-PELA-Ms meets the property requirement of controlled release, but the biological activity of released bFGF is destroyed partially.