Mai Gu1, Sophie Ghafari, Manxu Zhao. 1. Department of Pathology, University of California Irvine Medical Center, Orange, California 92868, USA. mgu@uci.edu
Abstract
OBJECTIVE: To assess the usefulness of fluorescence in situ hybridization (FISH) for HER-2/neu amplification of breast carcinoma in archival fine needle aspiration biopsy (FNAB) specimens. STUDY DESIGN: All FISH performed on formalin-fixed, paraffin-embedded surgical specimens during January 2003-August 2003 at the University of California Irvine Medical Center were selected. Prior FNABs were retrieved. One cytologic slide was destained in each case. The results were compared with those obtained on histologic specimens using the paired t test. RESULTS: FISH was performed on 41 surgical specimens of breast carcinoma. Thirteen patients had prior FNABs that were positive for adenocarcinoma. After hybridization on destained fine needle aspiration slides, no cells were found in 2 cases, and the results were not readable in 2 cases. In the remaining 9 cases, the results, expressed as the ratio of copies of the HER-2/neu gene to copies of the chromosome 17 centromere, were 5.10, 1.14, 1.21, 1.12, 0.74, 1.11, 1.21, 9.87 and 2.4. Results on the corresponding histologic specimens were 5.25, 1.05, 1.13, 1.22, 1.13, 1.12, 1.21, 9.35 and 2.61, respectively. No significant difference was found (p = 0.23). CONCLUSION: HER-2/neu amplification status by FISH can be accurately and reliably evaluated in existing archival cytologic slides.
OBJECTIVE: To assess the usefulness of fluorescence in situ hybridization (FISH) for HER-2/neu amplification of breast carcinoma in archival fine needle aspiration biopsy (FNAB) specimens. STUDY DESIGN: All FISH performed on formalin-fixed, paraffin-embedded surgical specimens during January 2003-August 2003 at the University of California Irvine Medical Center were selected. Prior FNABs were retrieved. One cytologic slide was destained in each case. The results were compared with those obtained on histologic specimens using the paired t test. RESULTS: FISH was performed on 41 surgical specimens of breast carcinoma. Thirteen patients had prior FNABs that were positive for adenocarcinoma. After hybridization on destained fine needle aspiration slides, no cells were found in 2 cases, and the results were not readable in 2 cases. In the remaining 9 cases, the results, expressed as the ratio of copies of the HER-2/neu gene to copies of the chromosome 17 centromere, were 5.10, 1.14, 1.21, 1.12, 0.74, 1.11, 1.21, 9.87 and 2.4. Results on the corresponding histologic specimens were 5.25, 1.05, 1.13, 1.22, 1.13, 1.12, 1.21, 9.35 and 2.61, respectively. No significant difference was found (p = 0.23). CONCLUSION: HER-2/neu amplification status by FISH can be accurately and reliably evaluated in existing archival cytologic slides.
Authors: Laura E Moreno Luna; Benjamin Kipp; Kevin C Halling; Thomas J Sebo; Walter K Kremers; Lewis R Roberts; Emily G Barr Fritcher; Michael J Levy; Gregory J Gores Journal: Gastroenterology Date: 2006-08-16 Impact factor: 22.682
Authors: Coya Tapia; Spasenija Savic; Urs Wagner; René Schönegg; Hedvika Novotny; Bruno Grilli; Michelle Herzog; Audrey Devito Barascud; Inti Zlobec; Gieri Cathomas; Luigi Terracciano; Georg Feichter; Lukas Bubendorf Journal: Breast Cancer Res Date: 2007 Impact factor: 6.466
Authors: S Savic; C Tapia; B Grilli; A Rufle; M P Bihl; A de Vito Barascud; M Herzog; L Terracciano; F Baty; L Bubendorf Journal: Br J Cancer Date: 2007-12-18 Impact factor: 7.640