Literature DB >> 16333118

Detection and serotyping of Streptococcus pneumoniae from nasopharyngeal samples by PCR-based multiplex assay.

Jaime Moreno1, Elkin Hernández, Olga Sanabria, Elizabeth Castañeda.   

Abstract

We developed a multiplex PCR-based methodology for nasopharyngeal samples maintained in egg thioglycolate antibiotic and skim milk-tryptone-glucose-glycerol media to identify and serotype the most important serotypes of Streptococcus pneumoniae that cause invasive disease in children. This technique can be used to study the epidemiology of pneumococcal colonization and the effect of conjugate vaccines.

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Year:  2005        PMID: 16333118      PMCID: PMC1317176          DOI: 10.1128/JCM.43.12.6152-6154.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  20 in total

1.  Confirmation of psaA in all 90 serotypes of Streptococcus pneumoniae by PCR and potential of this assay for identification and diagnosis.

Authors:  K E Morrison; D Lake; J Crook; G M Carlone; E Ades; R Facklam; J S Sampson
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

2.  Lack of utility of serotyping multiple colonies for detection of simultaneous nasopharyngeal carriage of different pneumococcal serotypes.

Authors:  R E Huebner; R Dagan; N Porath; A D Wasas; K P Klugman
Journal:  Pediatr Infect Dis J       Date:  2000-10       Impact factor: 2.129

3.  Egg-based media for delayed processing of nasopharyngeal swabs in colonization studies of Streptococcus pneumoniae.

Authors:  B M Gray
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2002-09-13       Impact factor: 3.267

4.  Evaluation of semiautomated multiplex PCR assay for determination of Streptococcus pneumoniae serotypes and serogroups.

Authors:  Elliot R Lawrence; David B Griffiths; Siobhán A Martin; Robert C George; Lucinda M C Hall
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

5.  Evaluation of a medium (STGG) for transport and optimal recovery of Streptococcus pneumoniae from nasopharyngeal secretions collected during field studies.

Authors:  K L O'Brien; M A Bronsdon; R Dagan; P Yagupsky; J Janco; J Elliott; C G Whitney; Y H Yang; L G Robinson; B Schwartz; G M Carlone
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

6.  Nasopharyngeal carriage of Streptococcus pneumoniae in Finnish children younger than 2 years old.

Authors:  R K Syrjänen; T M Kilpi; T H Kaijalainen; E E Herva; A K Takala
Journal:  J Infect Dis       Date:  2001-07-06       Impact factor: 5.226

7.  Post-PCV7 changes in colonizing pneumococcal serotypes in 16 Massachusetts communities, 2001 and 2004.

Authors:  Susan S Huang; Richard Platt; Sheryl L Rifas-Shiman; Stephen I Pelton; Donald Goldmann; Jonathan A Finkelstein
Journal:  Pediatrics       Date:  2005-09       Impact factor: 7.124

8.  Inhibition of OM pathogens by alpha-hemolytic streptococci from healthy children, children with SOM and children with rAOM.

Authors:  K Tano; E Grahn-Håkansson; S E Holm; S Hellström
Journal:  Int J Pediatr Otorhinolaryngol       Date:  2000-12-22       Impact factor: 1.675

9.  Genetic diversity and clonal patterns among antibiotic-susceptible and -resistant Streptococcus pneumoniae colonizing children: day care centers as autonomous epidemiological units.

Authors:  R Sá-Leão; A Tomasz; I S Sanches; S Nunes; C R Alves; A B Avô; J Saldanha; K G Kristinsson; H de Lencastre
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

Review 10.  Efficacy of pneumococcal conjugate vaccines and their effect on carriage and antimicrobial resistance.

Authors:  K P Klugman
Journal:  Lancet Infect Dis       Date:  2001-09       Impact factor: 25.071

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  7 in total

1.  Molecular epidemiological investigation to determine the source of a fatal case of serotype 22F pneumococcal meningitis.

Authors:  Juliana Lamaro-Cardoso; Ana Paula S de Lemos; Maria da Glória Carvalho; Fabiana Cristina Pimenta; Alexis Roundtree; Lorena Motta; Maria Aparecida Vieira; Sabrina Sgambatti; Licia Kamila Thörn; Vicente Pessoa-Junior; Ruth Minamisava; Lee H Harrison; Bernard W Beall; Maria Cristina de Cunto Brandileone; Ana Lucia Andrade
Journal:  J Med Microbiol       Date:  2012-01-27       Impact factor: 2.472

2.  Serotyping of Streptococcus pneumoniae isolates from nasopharyngeal samples: use of an algorithm combining microbiologic, serologic, and sequential multiplex PCR techniques.

Authors:  Karen Miernyk; Carolynn Debyle; Marcella Harker-Jones; Kimberlee Boyd Hummel; Thomas Hennessy; Jay Wenger; Karen Rudolph
Journal:  J Clin Microbiol       Date:  2011-07-20       Impact factor: 5.948

3.  Effect of swab composition and use of swabs versus swab-containing skim milk-tryptone-glucose-glycerol (STGG) on culture- or PCR-based detection of Streptococcus pneumoniae in simulated and clinical respiratory specimens in STGG transport medium.

Authors:  Lorry G Rubin; Atqia Rizvi; Aryeh Baer
Journal:  J Clin Microbiol       Date:  2008-06-18       Impact factor: 5.948

4.  Dynamics of pneumococcal nasopharyngeal carriage in healthy children attending a day care center in northern Spain. Influence of detection techniques on the results.

Authors:  María Ercibengoa; Nerea Arostegi; José M Marimón; Marta Alonso; Emilio Pérez-Trallero
Journal:  BMC Infect Dis       Date:  2012-03-22       Impact factor: 3.090

5.  Differential effects of frozen storage on the molecular detection of bacterial taxa that inhabit the nasopharynx.

Authors:  Brenda A Kwambana; Nuredin I Mohammed; David Jeffries; Mike Barer; Richard A Adegbola; Martin Antonio
Journal:  BMC Clin Pathol       Date:  2011-01-24

6.  Microarray for serotyping of Bartonella species.

Authors:  Cyrille J Bonhomme; Claude Nappez; Didier Raoult
Journal:  BMC Microbiol       Date:  2007-06-25       Impact factor: 3.605

7.  Serotypes and Antibiotic Susceptibility of Streptococcus pneumoniae Isolates from Invasive Pneumococcal Disease and Asymptomatic Carriage in a Pre-vaccination Period, in Algeria.

Authors:  Hanifa Ziane; Vera Manageiro; Eugénia Ferreira; Inês B Moura; Soumia Bektache; Mohamed Tazir; Manuela Caniça
Journal:  Front Microbiol       Date:  2016-06-14       Impact factor: 5.640

  7 in total

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