Literature DB >> 16330186

Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copies.

Ralf Ehricht1, Peter Slickers, Stefanie Goellner, Helmut Hotzel, Konrad Sachse.   

Abstract

This study was conducted to determine the detection limit of an optimized DNA microarray assay for detection and species identification of chlamydiae. Examination of dilution series of a plasmid standard carrying the target sequence from Chlamydia trachomatis and genomic DNA of this organism revealed that a single PCR-amplifiable target copy was sufficient to obtain a specific hybridization pattern. This performance renders the test suitable for routine testing of clinical samples.

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Year:  2005        PMID: 16330186     DOI: 10.1016/j.mcp.2005.09.003

Source DB:  PubMed          Journal:  Mol Cell Probes        ISSN: 0890-8508            Impact factor:   2.365


  66 in total

1.  Detection of Chlamydia species in 2 cases of equine abortion in Switzerland: a retrospective study from 2000 to 2018.

Authors:  Sibylle Baumann; Corinne Gurtner; Hanna Marti; Nicole Borel
Journal:  J Vet Diagn Invest       Date:  2020-06-11       Impact factor: 1.279

2.  Chlamydiaceae in North Atlantic Seabirds Admitted to a Wildlife Rescue Center in Western France.

Authors:  R Aaziz; P Gourlay; F Vorimore; K Sachse; V I Siarkou; K Laroucau
Journal:  Appl Environ Microbiol       Date:  2015-05-01       Impact factor: 4.792

3.  Defining species-specific immunodominant B cell epitopes for molecular serology of Chlamydia species.

Authors:  K Shamsur Rahman; Erfan U Chowdhury; Anil Poudel; Anke Ruettger; Konrad Sachse; Bernhard Kaltenboeck
Journal:  Clin Vaccine Immunol       Date:  2015-03-11

4.  Fluorescence in situ hybridization in species-specific diagnosis of ovine Campylobacter abortions.

Authors:  Godelind A Wolf-Jäckel; Mette Boye; Øystein Angen; Matthias Müller; Tim K Jensen
Journal:  J Vet Diagn Invest       Date:  2020-04-10       Impact factor: 1.279

5.  Assessment of Chlamydia psittaci Shedding and Environmental Contamination as Potential Sources of Worker Exposure throughout the Mule Duck Breeding Process.

Authors:  V Hulin; P Bernard; F Vorimore; R Aaziz; D Cléva; J Robineau; B Durand; L Angelis; V I Siarkou; K Laroucau
Journal:  Appl Environ Microbiol       Date:  2015-12-28       Impact factor: 4.792

6.  Distinct intensity of host-pathogen interactions in Chlamydia psittaci- and Chlamydia abortus-infected chicken embryos.

Authors:  Maria Braukmann; Konrad Sachse; Ilse D Jacobsen; Martin Westermann; Christian Menge; Hans-Peter Saluz; Angela Berndt
Journal:  Infect Immun       Date:  2012-06-11       Impact factor: 3.441

7.  Ectopic lymphoid tissue formation in the lungs of mice infected with Chlamydia pneumoniae is associated with epithelial macrophage inflammatory protein-2/CXCL2 expression.

Authors:  P M Fitch; N M Wheelhouse; P Bowles; M Paterson; D Longbottom; G Entrican; S E M Howie
Journal:  Clin Exp Immunol       Date:  2010-09-14       Impact factor: 4.330

8.  A novel synthetic peptide microarray assay detects Chlamydia species-specific antibodies in animal and human sera.

Authors:  Konrad Sachse; Kh Shamsur Rahman; Christiane Schnee; Elke Müller; Madlen Peisker; Thomas Schumacher; Evelyn Schubert; Anke Ruettger; Bernhard Kaltenboeck; Ralf Ehricht
Journal:  Sci Rep       Date:  2018-03-16       Impact factor: 4.379

9.  Rapid identification of bacterial pathogens using a PCR- and microarray-based assay.

Authors:  Anna-Kaarina Järvinen; Sanna Laakso; Pasi Piiparinen; Anne Aittakorpi; Merja Lindfors; Laura Huopaniemi; Heli Piiparinen; Minna Mäki
Journal:  BMC Microbiol       Date:  2009-08-10       Impact factor: 3.605

10.  Transcriptional analysis of in vitro expression patterns of Chlamydophila abortus polymorphic outer membrane proteins during the chlamydial developmental cycle.

Authors:  Nicholas Wheelhouse; Kevin Aitchison; Lucy Spalding; Morag Livingstone; David Longbottom
Journal:  Vet Res       Date:  2009-05-21       Impact factor: 3.683

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