| Literature DB >> 1632526 |
A M Douglas1, A M Georgalis, L R Benton, K L Canavan, B A Atchison.
Abstract
A rapid nontoxic method for the purification of DNA from human leucocytes is described. Preliminary experiments which tested different methods of DNA purification indicated that digestion of proteins with proteinase K was unnecessary. This led to the development of a simple procedure involving lysis of the cells in SDS followed by extraction with 6 M NaCl. The method described overcomes the requirement for lengthy incubations in the presence of expensive proteinase K and subsequent extraction with toxic chemicals.Entities:
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Year: 1992 PMID： 1632526 DOI： 10.1016/0003-2697(92)90352-8
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365