Literature DB >> 1632487

A modified colorimetric method for the determination of orthophosphate in the presence of high ATP concentrations.

P González-Romo1, S Sánchez-Nieto, M Gavilanes-Ruíz.   

Abstract

We describe a modified colorimetric method that quantitates inorganic phosphate linearly up to 60 nmol, with high stability of the developed color and with a low interference by ATP concentration (up to 30 mM). This method is very suitable for use in ATPase enzymatic assays, especially with enzymes that have low specific activities and (or) high Km values for ATP.

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Year:  1992        PMID： 1632487     DOI： 10.1016/0003-2697(92)90458-j

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  22 in total

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10.  Aluminum Elicits Exocellular Phosphatidylethanolamine Production in Pseudomonas fluorescens.

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