OBJECTIVE: The lymphatic drainage from a tumour is received in the sentinel node where the immune system encounters tumour derived antigens. We investigated anti-tumoural lymphocyte function in sentinel nodes from patients with urinary bladder cancer. METHODS: In 14 patients undergoing cystectomy due to bladder cancer, radioactive tracer and blue dye were used to identify the sentinel node. Cell suspensions from the tumour, sentinel- and non-sentinel nodes and peripheral blood were analyzed by flow cytometry with antibodies against lymphocyte surface antigens and against the tumour cell marker cytokeratin-20. Reactivity against autologous tumour extract and the mitogen Concanavalin A was tested in proliferation assays with 3H-Thymidine incorporation. Lymphocytes were put in long-term culture with IL-2 and autologous tumour extract. RESULTS: Sentinel nodes were detected in 12 of the 14 patients. Antigen dependent proliferation in response to autologous tumour extract was detected in 6 patients, in 5 cases in sentinel nodes, in the remaining case in a non-sentinel node. Proliferation against Concanavalin A was vigorous in lymph nodes from all patients, whereas tumour infiltrating lymphocytes were unresponsive. Lymphocytes from sentinel nodes could be expanded in vitro. CONCLUSION: Tumour reactive lymphocytes are present in sentinel nodes draining human bladder cancers. These cells display immunologic function upon restimulation in vitro, and provide a promising source for expansion and subsequent adoptive T cell immunotherapy.
OBJECTIVE: The lymphatic drainage from a tumour is received in the sentinel node where the immune system encounters tumour derived antigens. We investigated anti-tumoural lymphocyte function in sentinel nodes from patients with urinary bladder cancer. METHODS: In 14 patients undergoing cystectomy due to bladder cancer, radioactive tracer and blue dye were used to identify the sentinel node. Cell suspensions from the tumour, sentinel- and non-sentinel nodes and peripheral blood were analyzed by flow cytometry with antibodies against lymphocyte surface antigens and against the tumour cell marker cytokeratin-20. Reactivity against autologous tumour extract and the mitogen Concanavalin A was tested in proliferation assays with 3H-Thymidine incorporation. Lymphocytes were put in long-term culture with IL-2 and autologous tumour extract. RESULTS: Sentinel nodes were detected in 12 of the 14 patients. Antigen dependent proliferation in response to autologous tumour extract was detected in 6 patients, in 5 cases in sentinel nodes, in the remaining case in a non-sentinel node. Proliferation against Concanavalin A was vigorous in lymph nodes from all patients, whereas tumour infiltrating lymphocytes were unresponsive. Lymphocytes from sentinel nodes could be expanded in vitro. CONCLUSION:Tumour reactive lymphocytes are present in sentinel nodes draining humanbladder cancers. These cells display immunologic function upon restimulation in vitro, and provide a promising source for expansion and subsequent adoptive T cell immunotherapy.
Authors: B E Schaafsma; F P R Verbeek; H W Elzevier; Q R J G Tummers; J R van der Vorst; J V Frangioni; C J H van de Velde; R C M Pelger; A L Vahrmeijer Journal: J Surg Oncol Date: 2014-08-11 Impact factor: 3.454
Authors: Mona Karlsson; Per Marits; Kjell Dahl; Tobias Dagöö; Sven Enerbäck; Magnus Thörn; Ola Winqvist Journal: Ann Surg Oncol Date: 2010-01-30 Impact factor: 5.344
Authors: Robert Rosenblatt; Markus Johansson; Farhood Alamdari; Alexander Sidiki; Benny Holmström; Johan Hansson; Janos Vasko; Per Marits; Susanne Gabrielsson; Katrine Riklund; Ola Winqvist; Amir Sherif Journal: World J Urol Date: 2016-10-13 Impact factor: 4.226
Authors: Kjell Dahl; Mona Karlsson; Per Marits; Anna Hoffstedt; Ola Winqvist; Magnus Thörn Journal: Ann Surg Oncol Date: 2008-02-26 Impact factor: 5.344