Ramin Alemzadeh1, Kathryn Tushaus. 1. Pediatric Endocrinology & Metabolism, Medical College of Wisconsin, Milwaukee, WI 53226, USA. ralemzad@mcw.edu
Abstract
BACKGROUND: Attenuation of hyperinsulinemia by diazoxide (DZ), an inhibitor of glucose-mediated insulin secretion, in Zucker diabetic fatty (ZDF) rats, an animal model of type 2 diabetes and leptin resistance, decreased weight gain, improved lipid profile and prevented diabetes. Since the opposing effects of insulin and leptin regulate hepatic lipogenesis, we studied effects of insulin suppression on key insulin-sensitive genes regulating hepatic lipogenesis. MATERIAL/ METHODS: DZ (150 mg/kg/day) or vehicle [pair-fed (PF) and control (C)] was administered to pre-diabetic obese ZDF and ZDF lean (ZL) rats for 8 weeks. RESULTS: Hepatic glucose transporter-2 protein expression decreased only in DZ-ZDF rats (p<0.001). However, insulin receptor substrate-1/2 protein expressions were enhanced in DZ-ZDF (p<0.02) and DZ-ZL (p<0.03) rats corresponding to increased phosphorylated protein kinase B levels in both strains (p<0.03). DZ increased glycogen synthase expression in ZDF (p<0.005) and ZL (p<0.002) rats. DZ reduced expression of sterol regulatory element-binding protein-1c, (p<0.0001), fatty acid synthase (p<0.002), acetyl CoA carboxylase (p<0.001), hormone-sensitive lipase (p<0.005), and peroxisome proliferator agonist receptor-gamma (p<0.02) in ZDF rats, without altering expressions of acyl CoA oxidase, peroxisome proliferator receptor-alpha, and carnitine palmitoyl transferase-1. DZ decreased hepatic triglycerides (p<0.001), long chain acyl-CoA (p<0.001) and cholesterol (p<0.01) contents only in ZDF rats, but increased glycogen content in both strains (p<0.02). CONCLUSIONS: Suppression of hyperinsulinemia by DZ enhanced hepatic insulin sensitivity and decreased expression of key genes regulating hepatic lipogenesis without altering genes regulating lipid oxidation; implying that attenuation of hyperinsulinemic state by DZ enhances metabolic efficiency of insulin and is therapeutically beneficial.
BACKGROUND: Attenuation of hyperinsulinemia by diazoxide (DZ), an inhibitor of glucose-mediated insulin secretion, in Zucker diabetic fatty (ZDF) rats, an animal model of type 2 diabetes and leptin resistance, decreased weight gain, improved lipid profile and prevented diabetes. Since the opposing effects of insulin and leptin regulate hepatic lipogenesis, we studied effects of insulin suppression on key insulin-sensitive genes regulating hepatic lipogenesis. MATERIAL/ METHODS:DZ (150 mg/kg/day) or vehicle [pair-fed (PF) and control (C)] was administered to pre-diabetic obese ZDF and ZDF lean (ZL) rats for 8 weeks. RESULTS: Hepatic glucose transporter-2 protein expression decreased only in DZ-ZDFrats (p<0.001). However, insulin receptor substrate-1/2 protein expressions were enhanced in DZ-ZDF (p<0.02) and DZ-ZL (p<0.03) rats corresponding to increased phosphorylated protein kinase B levels in both strains (p<0.03). DZ increased glycogen synthase expression in ZDF (p<0.005) and ZL (p<0.002) rats. DZ reduced expression of sterol regulatory element-binding protein-1c, (p<0.0001), fatty acid synthase (p<0.002), acetyl CoA carboxylase (p<0.001), hormone-sensitive lipase (p<0.005), and peroxisome proliferator agonist receptor-gamma (p<0.02) in ZDFrats, without altering expressions of acyl CoA oxidase, peroxisome proliferator receptor-alpha, and carnitine palmitoyl transferase-1. DZ decreased hepatic triglycerides (p<0.001), long chain acyl-CoA (p<0.001) and cholesterol (p<0.01) contents only in ZDFrats, but increased glycogen content in both strains (p<0.02). CONCLUSIONS: Suppression of hyperinsulinemia by DZ enhanced hepatic insulin sensitivity and decreased expression of key genes regulating hepatic lipogenesis without altering genes regulating lipid oxidation; implying that attenuation of hyperinsulinemic state by DZ enhances metabolic efficiency of insulin and is therapeutically beneficial.
Authors: Xabier Buqué; María José Martínez; Ainara Cano; María E Miquilena-Colina; Carmelo García-Monzón; Patricia Aspichueta; Begoña Ochoa Journal: J Lipid Res Date: 2009-09-24 Impact factor: 5.922
Authors: Manuel Rosety-Rodriguez; Ignacio Rosety; Gabriel Fornieles-Gonzalez; Antonio Jesus Diaz-Ordonez; Alejandra Camacho; Miguel Angel Rosety; Antonio Pardo; Manuel Rosety; Ramon Alvero; Francisco Javier Ordonez Journal: Med Sci Monit Date: 2012-09