Literature DB >> 16314968

Improved cellular response of osteoblast cells using recombinant human osteopontin protein produced by Escherichia coli.

Jun-Hyeog Jang1, Ji-Hyun Kim.   

Abstract

Osteopontin is a major non-collagenous bone matrix protein secreted into the mineralizing extracellular matrix by osteoblasts during bone development. Recombinant human osteopontin (hOPN) that includes the Arg-Gly-Asp (RGD) cell recognition site was expressed in Escherichia coli and the purified osteopontin increased cell adhesion, proliferation and differentiation of osteoblast cells (p<0.05).

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Year:  2005        PMID: 16314968     DOI: 10.1007/s10529-005-3551-6

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN: 0141-5492            Impact factor:   2.461


  5 in total

1.  The Novel-miR-659/SPP1 Interaction Regulates Fat Deposition in Castrated Male Pigs.

Authors:  Lianmei Xiao; Qiao Xu; Ximing Liu; Shuheng Chan; Yabiao Luo; Shuaihan He; Meiying Fang
Journal:  Animals (Basel)       Date:  2022-04-07       Impact factor: 3.231

2.  Expression and purification of non-tagged recombinant mouse SPP1 in E. coli and its biological significance.

Authors:  Shunyan Weng; Liang Zhou; Lei Han; Yunsheng Yuan
Journal:  Bioengineered       Date:  2014-10-30       Impact factor: 3.269

3.  Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts.

Authors:  Douglas E Rodriguez; Taili Thula-Mata; Edgardo J Toro; Ya-Wen Yeh; Carl Holt; L Shannon Holliday; Laurie B Gower
Journal:  Acta Biomater       Date:  2013-10-17       Impact factor: 8.947

4.  Recombinant human osteopontin expressed in Nicotiana benthamiana stimulates osteogenesis related genes in human periodontal ligament cells.

Authors:  Kaewta Rattanapisit; Supaniga Abdulheem; Daneeya Chaikeawkaew; Anchanee Kubera; Hugh S Mason; Julian K-C Ma; Prasit Pavasant; Waranyoo Phoolcharoen
Journal:  Sci Rep       Date:  2017-12-11       Impact factor: 4.379

5.  Separation Options for Phosphorylated Osteopontin from Transgenic Microalgae Chlamydomonas reinhardtii.

Authors:  Ayswarya Ravi; Shengchun Guo; Beth Rasala; Miller Tran; Stephen Mayfield; Zivko L Nikolov
Journal:  Int J Mol Sci       Date:  2018-02-16       Impact factor: 5.923

  5 in total

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