Microbiological investigations for sanitary assessment of wastewater treated in constructed wetlands.

Microbiological investigations into the occurrence and fate of pathogens in wastewater were carried out in a multi-annual measurement programme, using samples from three artificial wetlands equipped with pre-treatment systems (multi-chamber septic tanks, lagoons) and predominantly treating domestic wastewater. In this study, the concentrations of indicator organisms and pathogenic or potentially pathogenic microorganisms were determined in the various components or stages of the systems. The evaluation of data from some 3.600 microbiological analyses and a comparison with older data from a plant which has already been operation for 18 y made it possible for the first time ever to include operational factors in the assessment. Average removal of all major indicator organisms and pathogens is 1.5-2.5 log units for one-stage systems and increases to 3-5 log units for multi-stage systems. Significant differences between horizontal and vertical filters were not found. If no clogging problems arise during their operation, multi-stage systems can meet the requirements of the EU bathing water and irrigation water directives. Influent concentration, wastewater temperature, and hydraulic loading rate were found to be major factors of influence. The performance of constructed wetlands in removing microorganisms is clearly superior to that of conventional biological activated sludge systems.