| Literature DB >> 16311514 |
C Krakstad1, L Herfindal, B T Gjertsen, R Bøe, O K Vintermyr, K E Fladmark, S O Døskeland.
Abstract
The protein phosphatase inhibitor microcystin-LR (MC) induced hepatocyte apoptosis mediated by the calcium-calmodulin-dependent multifunctional protein kinase II (CaMKII). CaMKII antagonists were added at various times after MC to define for how long the cells depended on CaMKII activity to be committed to execute the various parameters of death. Shrinkage and nonpolarized budding were reversible and not coupled to commitment. A critical commitment step was observed 15-20 min after MC (0.5 microM) addition. After this, CaMKII inhibitors no longer protected against polarized budding, DNA fragmentation, lost protein synthesis capability, and cell disruption. Commitment to chromatin hypercondensation occurred 40 min after MC addition. In conclusion, irreversible death commitment was coupled to polarized budding, but not to shrinkage or chromatin condensation. Antioxidant prevented chromatin condensation when given after the CaMKII-dependent commitment point, suggesting that CaMKII had mediated the accumulation of a second messenger of reactive oxygen species nature.Entities:
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Year: 2005 PMID： 16311514 DOI： 10.1038/sj.cdd.4401798
Source DB: PubMed Journal: Cell Death Differ ISSN： 1350-9047 Impact factor: 15.828