Literature DB >> 1631098

Structures of apo and complexed Escherichia coli glycinamide ribonucleotide transformylase.

R J Almassy1, C A Janson, C C Kan, Z Hostomska.   

Abstract

The three-dimensional structure of phosphoribosylglycinamide formyltransferase (10-formyltetrahydrofolate:5'-phosphoribosylglycinamide formyltransferase, EC 2.1.2.2) has been solved both as an apoenzyme at 2.8-A resolution and as a ternary complex with the substrate glycinamide ribonucleotide and a folate inhibitor at 2.5-A resolution. The structure is a modified doubly wound alpha/beta sheet with flexibility in the active site, including a disordered loop in the apo structure, which is ordered in the ternary complex structure. This enzyme is a target for anti-cancer therapy and now for structure-based drug design.

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Year:  1992        PMID： 1631098      PMCID： PMC49448          DOI： 10.1073/pnas.89.13.6114

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  24 in total

1.  Intensity-based domain refinement of oriented but unpositioned molecular replacement models.

Authors:  T O Yeates; J M Rini
Journal:  Acta Crystallogr A       Date:  1990-05-01       Impact factor: 2.290

2.  Stereochemically restrained refinement of macromolecular structures.

Authors:  W A Hendrickson
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

3.  Synthesis of the antileukemic agents 5,10-dideazaaminopterin and 5,10-dideaza-5,6,7,8-tetrahydroaminopterin.

Authors:  E C Taylor; P J Harrington; S R Fletcher; G P Beardsley; R G Moran
Journal:  J Med Chem       Date:  1985-07       Impact factor: 7.446

4.  Resolution of phase ambiguity in macromolecular crystallography.

Authors:  B C Wang
Journal:  Methods Enzymol       Date:  1985       Impact factor: 1.600

5.  Active-site mapping and site-specific mutagenesis of glycinamide ribonucleotide transformylase from Escherichia coli.

Authors:  J Inglese; J M Smith; S J Benkovic
Journal:  Biochemistry       Date:  1990-07-17       Impact factor: 3.162

6.  The 6S- and 6R-diastereomers of 5, 10-dideaza-5, 6, 7, 8-tetrahydrofolate are equiactive inhibitors of de novo purine synthesis.

Authors:  R G Moran; S W Baldwin; E C Taylor; C Shih
Journal:  J Biol Chem       Date:  1989-12-15       Impact factor: 5.157

7.  Massive overproduction of dihydrofolate reductase in bacteria as a response to the use of trimethoprim.

Authors:  J Flensburg; O Sköld
Journal:  Eur J Biochem       Date:  1987-02-02

8.  Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12.

Authors:  J M Smith; H A Daum
Journal:  J Biol Chem       Date:  1987-08-05       Impact factor: 5.157

9.  Mammalian glycinamide ribonucleotide transformylase. Kinetic mechanism and associated de novo purine biosynthetic activities.

Authors:  C A Caperelli
Journal:  J Biol Chem       Date:  1989-03-25       Impact factor: 5.157

10.  Preliminary crystallographic investigations of glycinamide ribonucleotide transformylase.

Authors:  E A Stura; D L Johnson; J Inglese; J M Smith; S J Benkovic; I A Wilson
Journal:  J Biol Chem       Date:  1989-06-05       Impact factor: 5.157
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  37 in total

1.  Combinatorial protein engineering by incremental truncation.

Authors:  M Ostermeier; A E Nixon; J H Shim; S J Benkovic
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-30       Impact factor: 11.205

2.  Predicting flexible loop regions that interact with ligands: the challenge of accurate scoring.

Authors:  Matthew L Danielson; Markus A Lill
Journal:  Proteins       Date:  2011-11-09

3.  On the structural and functional modularity of glycinamide ribonucleotide formyltransferases.

Authors:  Seung-Goo Lee; Stefan Lutz; Stephen J Benkovic
Journal:  Protein Sci       Date:  2003-10       Impact factor: 6.725

4.  Synthetic cycle of the initiation module of a formylating nonribosomal peptide synthetase.

Authors:  Janice M Reimer; Martin N Aloise; Paul M Harrison; T Martin Schmeing
Journal:  Nature       Date:  2016-01-14       Impact factor: 49.962

5.  Structure and function of both domains of ArnA, a dual function decarboxylase and a formyltransferase, involved in 4-amino-4-deoxy-L-arabinose biosynthesis.

Authors:  Gareth J Williams; Steven D Breazeale; Christian R H Raetz; James H Naismith
Journal:  J Biol Chem       Date:  2005-04-04       Impact factor: 5.157

6.  Crystal structure of methionyl-tRNAfMet transformylase complexed with the initiator formyl-methionyl-tRNAfMet.

Authors:  E Schmitt; M Panvert; S Blanquet; Y Mechulam
Journal:  EMBO J       Date:  1998-12-01       Impact factor: 11.598

7.  Native-state conformational dynamics of GART: a regulatory pH-dependent coil-helix transition examined by electrostatic calculations.

Authors:  D Morikis; A H Elcock; P A Jennings; J A McCammon
Journal:  Protein Sci       Date:  2001-11       Impact factor: 6.725

8.  Proton transfer dynamics of GART: the pH-dependent catalytic mechanism examined by electrostatic calculations.

Authors:  D Morikis; A H Elcock; P A Jennings; J A McCammon
Journal:  Protein Sci       Date:  2001-11       Impact factor: 6.725

9.  The hydrogenase gene cluster of Rhizobium leguminosarum bv. viciae contains an additional gene (hypX), which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity.

Authors:  L Rey; D Fernández; B Brito; Y Hernando; J M Palacios; J Imperial; T Ruiz-Argüeso
Journal:  Mol Gen Genet       Date:  1996-09-13

10.  Automated protein motif generation in the structure-based protein function prediction tool ProMOL.

Authors:  Mikhail Osipovitch; Mitchell Lambrecht; Cameron Baker; Shariq Madha; Jeffrey L Mills; Paul A Craig; Herbert J Bernstein
Journal:  J Struct Funct Genomics       Date:  2015-11-16
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