OBJECTIVES: To produce a monoclonal antibody (MAb) against electronegative LDL (LDL-) for detecting this modified lipoprotein in blood plasma and tissues. DESIGN AND METHODS: LDL- was isolated from human blood plasma and used as an antigen for immunization of Balb/c mice. Lymphocytes of immunized mice were fused with myeloma cells (SP2/0) to obtain the hybridomas. LDL- was detected in blood plasma and atherosclerotic lesions of humans and rabbits by MAb-based ELISA and immunohistochemistry, respectively. RESULTS: LDL- concentrations were higher (P < 0.05) in the blood plasma of hypercholesterolemic subjects (HC, 248 +/- 77 mg/dL of total cholesterol) than in normolipidemic subjects (NL, 173 +/- 82 mg/dL of total cholesterol) and rabbits (HC, 250 +/- 15 mg/dL of cholesterol versus NL, 81 +/- 12 mg/dL of cholesterol). Moreover, LDL- was detected in the atherosclerotic lesions of humans and rabbits. CONCLUSION: These MAb-based immunoassays are adequate to detect LDL- in biological samples and represent an important tool for investigating the role of LDL- in atherosclerosis.
OBJECTIVES: To produce a monoclonal antibody (MAb) against electronegative LDL (LDL-) for detecting this modified lipoprotein in blood plasma and tissues. DESIGN AND METHODS: LDL- was isolated from human blood plasma and used as an antigen for immunization of Balb/c mice. Lymphocytes of immunized mice were fused with myeloma cells (SP2/0) to obtain the hybridomas. LDL- was detected in blood plasma and atherosclerotic lesions of humans and rabbits by MAb-based ELISA and immunohistochemistry, respectively. RESULTS: LDL- concentrations were higher (P < 0.05) in the blood plasma of hypercholesterolemic subjects (HC, 248 +/- 77 mg/dL of total cholesterol) than in normolipidemic subjects (NL, 173 +/- 82 mg/dL of total cholesterol) and rabbits (HC, 250 +/- 15 mg/dL of cholesterol versus NL, 81 +/- 12 mg/dL of cholesterol). Moreover, LDL- was detected in the atherosclerotic lesions of humans and rabbits. CONCLUSION: These MAb-based immunoassays are adequate to detect LDL- in biological samples and represent an important tool for investigating the role of LDL- in atherosclerosis.
Authors: Shang-Hung Chang; Michael Johns; Joseph J Boyle; Ellen McConnell; Paul A Kirkham; Colin Bicknell; M Zahoor-ul-Hassan Dogar; Robert J Edwards; Oliver Gale-Grant; Ramzi Khamis; Kurrun V V Ramkhelawon; Dorian O Haskard Journal: Hybridoma (Larchmt) Date: 2012-04
Authors: Ming-Yi Shen; Jing-Fang Hsu; Fang-Yu Chen; Jonathan Lu; Chia-Ming Chang; Mohammad Madjid; Juliette Dean; Richard A F Dixon; Steven Shayani; Tzu-Chieh Chou; Chu-Huang Chen Journal: J Clin Med Date: 2019-08-09 Impact factor: 4.241
Authors: Tanize do Espirito Santo Faulin; Soraya Megumi Kazuma; Gustavo Luis Tripodi; Marcela Frota Cavalcante; Felipe Wakasuqui; Cristiano Luis Pinto Oliveira; Maximilia Frazão de Souza Degenhardt; Jussara Michaloski; Ricardo José Giordano; Daniel Francisco Jacon Ketelhuth; Dulcineia Saes Parra Abdalla Journal: Biomolecules Date: 2019-08-20