Literature DB >> 16310421

Detection and quantification of ginsenoside Re in ginseng samples by a chromatographic immunostaining method using monoclonal antibody against ginsenoside Re.

Osamu Morinaga1, Hiroyuki Tanaka, Yukihiro Shoyama.   

Abstract

A chromatographic immunostaining method has been developed for the determination of ginsenoside Re (G-Re) in ginseng samples on a polyethersulphone (PES) membrane. G-Re standard and the extracts of ginseng roots were applied to a PES membrane and developed by methanol-water-acetic acid (45:55:1, by volume). G-Re was clearly detected by an immunostaining method using a monoclonal antibody against G-Re. The coloring spots of G-Re were analyzed quantitatively using NIH Image software indicating at least 0.125 microg of G-Re was detectable. G-Re can be analyzed quantitatively between 0.25 and 4.0 microg.

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Year:  2005        PMID： 16310421     DOI： 10.1016/j.jchromb.2005.10.040

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  9 in total

1.  Quantitative analysis of ginsenosides Rb1, Rg1, and Re in American ginseng berry and flower samples by ELISA using monoclonal antibodies.

Authors:  Boonchoo Sritularak; Osamu Morinaga; Chun-Su Yuan; Yukihiro Shoyama; Hiroyuki Tanaka
Journal:  J Nat Med       Date:  2009-04-18       Impact factor: 2.343

Review 2.  Isolation and analysis of ginseng: advances and challenges.

Authors:  Lian-Wen Qi; Chong-Zhi Wang; Chun-Su Yuan
Journal:  Nat Prod Rep       Date:  2011-01-24       Impact factor: 13.423

3.  Single-chain variable fragment antibody against ginsenoside Re as an effective tool for the determination of ginsenosides in various ginsengs.

Authors:  Benyakan Pongkitwitoon; Seiichi Sakamoto; Osamu Morinaga; Thaweesak Juengwatanatrakul; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal:  J Nat Med       Date:  2010-08-14       Impact factor: 2.343

4.  Pharmacology of ginsenosides: a literature review.

Authors:  Kar Wah Leung; Alice Sze-Tsai Wong
Journal:  Chin Med       Date:  2010-06-11       Impact factor: 5.455

5.  Application of Monoclonal Antibodies against Bioactive Natural Products: Eastern Blotting and Preparation of Knockout Extract.

Authors:  Hiroyuki Tanaka; Osamu Morinaga; Takuhiro Uto; Shunsuke Fuji; Frederick Asare Aboagye; Nguyen Huu Tung; Xiao Wei Li; Waraporn Putalun; Yukihiro Shoyama
Journal:  Int J Anal Chem       Date:  2012-02-14       Impact factor: 1.885

Review 6.  Monoclonal Antibodies and Immunoassay for Medical Plant-Derived Natural Products: A Review.

Authors:  Xin Yan; Yan Zhao; Yue Zhang; Huihua Qu
Journal:  Molecules       Date:  2017-02-26       Impact factor: 4.411

7.  PEI-assisted boronate affinity magnetic nanoparticle-based SELEX for efficient in vitro evolution of saponin-binding aptamers.

Authors:  Hui Zhang; Xue Li; Ailan Huang; Zhifeng Yan; Yang Chen; Zijun Bie
Journal:  RSC Adv       Date:  2021-02-25       Impact factor: 3.361

Review 8.  Immunological Separation of Bioactive Natural Compounds from Crude Drug Extract and Its Application for Cell-Based Studies.

Authors:  Takuhiro Uto; Tomoe Ohta; Shunsuke Fujii; Yukihiro Shoyama
Journal:  Antibodies (Basel)       Date:  2021-12-06

Review 9.  Enzyme-linked immunosorbent assay for the quantitative/qualitative analysis of plant secondary metabolites.

Authors:  Seiichi Sakamoto; Waraporn Putalun; Sornkanok Vimolmangkang; Waranyoo Phoolcharoen; Yukihiro Shoyama; Hiroyuki Tanaka; Satoshi Morimoto
Journal:  J Nat Med       Date:  2017-11-21       Impact factor: 2.343
  9 in total

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