| Literature DB >> 16309751 |
Gabriella Elia1, Nicola Decaro, Vito Martella, Marco Campolo, Costantina Desario, Eleonora Lorusso, Francesco Cirone, Canio Buonavoglia.
Abstract
A real-time PCR assay was developed for detection and quantitation of equid herpesvirus type 1 (EHV-1). The sensitivity of the assay was compared with an established nested-PCR (n-PCR). The real-time PCR detected 1 copy of target DNA, with a sensitivity 1 log higher than gel-based n-PCR. The assay was able to detect specifically EHV-1 DNA in equine tissue samples and there was no cross-amplification of other horse herpesviruses. Real-time PCR was applied to determine EHV-1 load in tissue samples from equine aborted fetuses. The high sensitivity and reproducibility of the EHV-1-specific fluorogenic PCR assay, combined with the wide dynamic range and the high throughput, make this method suitable for diagnostic and research applications.Entities:
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Year: 2005 PMID： 16309751 DOI： 10.1016/j.jviromet.2005.10.024
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014