Hong Deng1, Fei Zou, Hai-ji Luo. 1. College of Tropical Medicine, Southern Medical University, Guangzhou 510515, China. denghong-gzh@163.com
Abstract
OBJECTIVE: To investigate the role of all-trans retinoic acid (atRA) in inducing differentiation of neonatal rat striatal neural stem cells (NSCs). METHODS: Neonatal rat striatal NSCs were obtained by mechanical isolation and serum-free culture. The roles of atRA at different concentrations in inducing the differentiation of NSCs were observed by immunofluorescent cytochemical staining, and the expression of retinoic acid receptor (RAR) gene was determined by semi-quantitative RT-PCR. RESULTS: atRA could dose-dependently accelerate the differentiation of NSCs into neuron-like cells, and the physiological concentration of atRA was optimal for inducing NSC differentiation. atRA could induce the expression of RARbeta mRNA in a dose- and time-dependent manner. CONCLUSION: atRA can accelerate differentiation of NSCs into neuron-likes cells and up-regulate the expression of RAR-beta mRNA in neonatal rat striatal NSCs.
OBJECTIVE: To investigate the role of all-trans retinoic acid (atRA) in inducing differentiation of neonatal rat striatal neural stem cells (NSCs). METHODS: Neonatal rat striatal NSCs were obtained by mechanical isolation and serum-free culture. The roles of atRA at different concentrations in inducing the differentiation of NSCs were observed by immunofluorescent cytochemical staining, and the expression of retinoic acid receptor (RAR) gene was determined by semi-quantitative RT-PCR. RESULTS:atRA could dose-dependently accelerate the differentiation of NSCs into neuron-like cells, and the physiological concentration of atRA was optimal for inducing NSC differentiation. atRA could induce the expression of RARbeta mRNA in a dose- and time-dependent manner. CONCLUSION:atRA can accelerate differentiation of NSCs into neuron-likes cells and up-regulate the expression of RAR-beta mRNA in neonatal rat striatal NSCs.