| Literature DB >> 16289902 |
Emma J Parkin1, Thirsa Kraayenbrink, George L van Driem, Karma Tshering Of Gaselô, Peter de Knijff, Mark A Jobling.
Abstract
26 Y chromosome short tandem repeat (STR) loci were amplified in a sample of 856 unrelated males from Bhutan, using two multiplex polymerase chain reaction (PCR) assays. The first multiplex is the Y-STR 20plex described by Butler et al. [J.M. Butler, R. Schoske, P.M. Vallone, M.C. Kline, A.J. Redd, M.F. Hammer, A novel multiplex for simultaneous amplification of 20 Y chromosome STR markers, Forensic Sci. Int. 129 (2002) 10-24], and the second is a novel (but overlapping) 14plex that targets six additional Y-STRs (DYS425, DYS434, DYS435, DYS436, DYS461, DYS462) and also amplifies the amelogenin locus. The 26-loci give a discriminating power of 0.9957, though even at this resolution one haplotype occurs 24 times. We identify novel alleles at five loci and microvariants at a further three, which were characterised by sequencing. Extended (11-locus) haplotypes for these samples have been submitted to the Y-STR Haplotype Reference Database (YHRD).Entities:
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Year: 2005 PMID： 16289902 DOI： 10.1016/j.forsciint.2005.10.008
Source DB: PubMed Journal: Forensic Sci Int ISSN： 0379-0738 Impact factor: 2.395