| Literature DB >> 16289436 |
Kelath Murali Manoj1, Lowell P Hager.
Abstract
A method of detecting and assaying the halogenating activity of hemeperoxidases using the colored substrate, thionin, is reported here. In the presence of suitable amounts of peroxide and chloride, chloroperoxidase chlorinates thionin and bleaches the intense color of this substrate. The kinetics was quite similar to that of the established monochlorodimedone assay. Therefore, the thionin assay can be taken as an index of chlorinating activity. This reaction affords an escape from background problems and allows easy processing of large volumes of samples.Entities:
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Year: 2005 PMID： 16289436 DOI： 10.1016/j.ab.2005.10.014
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365