OBJECTIVE: To evaluated the cytotoxicity of a new nano-hydroxyapatite (n-HA) root canal sealer. METHODS: In this study, the cytotoxicity was evaluated by the method of MTT assay in vitro, and culture medium F12 as control, three concentrations of the soaking material cultured with mouse osteoblast separately, to test the cell relative growth rate (RGR) of every group. RESULTS: The toxicity graduation of the n-HA root canal sealer tends to 0 with the culture time increasing. The cell survival rate of n-HA root canal sealer showed high relatively. The OD value of cell was similar for the negative control and the extracts (P > 0.05). CONCLUSION: The result indicated that n-HA root canal sealer was compatible with the testcells.
OBJECTIVE: To evaluated the cytotoxicity of a new nano-hydroxyapatite (n-HA) root canal sealer. METHODS: In this study, the cytotoxicity was evaluated by the method of MTT assay in vitro, and culture medium F12 as control, three concentrations of the soaking material cultured with mouse osteoblast separately, to test the cell relative growth rate (RGR) of every group. RESULTS: The toxicity graduation of the n-HA root canal sealer tends to 0 with the culture time increasing. The cell survival rate of n-HA root canal sealer showed high relatively. The OD value of cell was similar for the negative control and the extracts (P > 0.05). CONCLUSION: The result indicated that n-HA root canal sealer was compatible with the testcells.