Z G Wang1, D B Durand, M Schoenberg, Y T Pan. 1. Department of Biomedical Engineering, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
Abstract
PURPOSE: We describe the technique of fluorescence image guided optical coherence tomography (FG-OCT). We examined its ability to enhance specificity and sensitivity for the noninvasive diagnosis of early bladder cancer. MATERIALS AND METHODS: Transitional cell carcinoma was developed in 54 Fisher 344 female rats by intravesical methyl-nitroso-urea instillations. Two or three rats were diagnosed sequentially by 5-ALA (5-aminolevulinic acid hydrochloride) induced fluorescence imaging, cross-sectional OCT and histological microscopy weekly during weeks 11 to 33 following initial methyl-nitroso-urea instillation to track the course of carcinogenesis. RESULTS: The specificity of fluorescence detection was significantly enhanced by FG-OCT (53% and 93%, respectively, p <0.0001). The sensitivity of fluorescence detection and FG-OCT was 79% and 100%, respectively. CONCLUSIONS: FG-OCT cystoscopy has the potential to diagnose early bladder cancer with high sensitivity and specificity with drastically decreased imaging time compared to that of white light guided OCT cystoscopy.
PURPOSE: We describe the technique of fluorescence image guided optical coherence tomography (FG-OCT). We examined its ability to enhance specificity and sensitivity for the noninvasive diagnosis of early bladder cancer. MATERIALS AND METHODS: Transitional cell carcinoma was developed in 54 Fisher 344 female rats by intravesical methyl-nitroso-urea instillations. Two or three rats were diagnosed sequentially by 5-ALA (5-aminolevulinic acid hydrochloride) induced fluorescence imaging, cross-sectional OCT and histological microscopy weekly during weeks 11 to 33 following initial methyl-nitroso-urea instillation to track the course of carcinogenesis. RESULTS: The specificity of fluorescence detection was significantly enhanced by FG-OCT (53% and 93%, respectively, p <0.0001). The sensitivity of fluorescence detection and FG-OCT was 79% and 100%, respectively. CONCLUSIONS: FG-OCT cystoscopy has the potential to diagnose early bladder cancer with high sensitivity and specificity with drastically decreased imaging time compared to that of white light guided OCT cystoscopy.
Authors: Hugang Ren; Wayne C Waltzer; Rahuldev Bhalla; Jingxuan Liu; Zhijia Yuan; Christopher S D Lee; Frank Darras; David Schulsinger; Howard L Adler; Jason Kim; Alek Mishail; Yingtian Pan Journal: Urology Date: 2009-08-05 Impact factor: 2.649
Authors: Sebina Shrestha; Michael J Serafino; Jesus Rico-Jimenez; Jesung Park; Xi Chen; Siqin Zhaorigetu; Brian L Walton; Javier A Jo; Brian E Applegate Journal: Biomed Opt Express Date: 2016-08-01 Impact factor: 3.732
Authors: Javier A Jo; Brian E Applegate; Jesung Park; Sebina Shrestha; Paritosh Pande; Irma B Gimenez-Conti; Jimi L Brandon Journal: IEEE Trans Biomed Eng Date: 2010-07-23 Impact factor: 4.538
Authors: Shuai Yuan; Celeste A Roney; Jeremiah Wierwille; Chao-Wei Chen; Biying Xu; Gary Griffiths; James Jiang; Hongzhou Ma; Alex Cable; Ronald M Summers; Yu Chen Journal: Phys Med Biol Date: 2010-01-07 Impact factor: 3.609