| Literature DB >> 16280176 |
Joshua O Ojwang1, Yan-Hong Wang, Philip R Wyde, Nikolaus H Fischer, Wolfgang Schuehly, James R Appleman, Soreeta Hinds, Craig D Shimasaki.
Abstract
A novel low molecular weight compound, CJ 4-16-4, isolated from ethnobotanicals using bioassay-guided fractionation, was found to be a potent inhibitor of respiratory syncytial virus (RSV) in vitro and in vivo. In vitro, a very low micromolar efficacious dose was obtained against at least four of subtype A (RSV-Long, RSV A2, and RSV A6 57754) and one of subtype B (Washington) RSV strains without seeing any significant cytotoxicity to Hep-2, MDCK or Vero cell lines. The drug inhibits growth of RSV in Hep-2 cells maintained in tissue culture at a very low concentration (approximately 0.07 microM) with cell toxicity >400 microM (TI>5880). In a cotton rat model of RSV infection, the drug was able to reduce viral titers by approximately 1 log at dose 12.5 and 25 mg/kg/day, and by >2 log at 100 mg/kg/day. This antiviral activity was specific as influenza A and B and herpes simplex 1 and 2 viruses were not inhibited. The results obtained indicate that CJ 4-16-4 warrants clinical development.Entities:
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Year: 2005 PMID： 16280176 DOI： 10.1016/j.antiviral.2005.09.003
Source DB: PubMed Journal: Antiviral Res ISSN： 0166-3542 Impact factor: 5.970