Literature DB >> 16278463

The NIT1 promoter allows inducible and reversible silencing of centrin in Chlamydomonas reinhardtii.

Bettina Koblenz1, Karl-Ferdinand Lechtreck.   

Abstract

An inverted repeat corresponding to parts of the centrin gene of Chlamydomonas reinhardtii was placed downstream of the NIT1 promoter, which is induced by ammonium starvation. After induction, transformants developed centrin deficiency as assayed by immunofluorescence, Western blotting, and Northern blotting. The effect was reversible, demonstrating that the NIT1 promoter allowed controlled RNA interference in Chlamydomonas reinhardtii.

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Year:  2005        PMID： 16278463      PMCID： PMC1287850          DOI： 10.1128/EC.4.11.1959-1962.2005

Source DB:  PubMed          Journal:  Eukaryot Cell        ISSN： 1535-9786


  13 in total

1.  Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA.

Authors:  H Shi; A Djikeng; T Mark; E Wirtz; C Tschudi; E Ullu
Journal:  RNA       Date:  2000-07       Impact factor: 4.942

2.  Centrin deficiency in Chlamydomonas causes defects in basal body replication, segregation and maturation.

Authors:  Bettina Koblenz; Jutta Schoppmeier; Andrea Grunow; Karl-Ferdinand Lechtreck
Journal:  J Cell Sci       Date:  2003-05-13       Impact factor: 5.285

3.  Transcriptional regulation of the Nia1 gene encoding nitrate reductase in Chlamydomonas reinhardtii: effects of various environmental factors on the expression of a reporter gene under the control of the Nia1 promoter.

Authors:  R Loppes; M Radoux; M C Ohresser; R F Matagne
Journal:  Plant Mol Biol       Date:  1999-11       Impact factor: 4.076

4.  Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.

Authors:  D S Gorman; R P Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1965-12       Impact factor: 11.205

5.  Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii.

Authors:  E Fernández; R Schnell; L P Ranum; S C Hussey; C D Silflow; P A Lefebvre
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

6.  Tandem inverted repeat system for selection of effective transgenic RNAi strains in Chlamydomonas.

Authors:  Jennifer Rohr; Nandita Sarkar; Susan Balenger; Byeong-ryool Jeong; Heriberto Cerutti
Journal:  Plant J       Date:  2004-11       Impact factor: 6.417

7.  GFP as a tool for the analysis of proteins in the flagellar basal apparatus of Chlamydomonas.

Authors:  Jutta Schoppmeier; Wolfgang Mages; Karl-Ferdinand Lechtreck
Journal:  Cell Motil Cytoskeleton       Date:  2005-08

8.  Inducible, reversible, and stable RNA interference in mammalian cells.

Authors:  Sunita Gupta; Rebecca A Schoer; James E Egan; Gregory J Hannon; Vivek Mittal
Journal:  Proc Natl Acad Sci U S A       Date:  2004-02-04       Impact factor: 11.205

9.  Temporal and spatial control of gene silencing in transgenic plants by inducible expression of double-stranded RNA.

Authors:  Shuai Chen; Daniel Hofius; Uwe Sonnewald; Frederik Börnke
Journal:  Plant J       Date:  2003-12       Impact factor: 6.417

10.  The abundant retinal protein of the Chlamydomonas eye is not the photoreceptor for phototaxis and photophobic responses.

Authors:  M Fuhrmann; A Stahlberg; E Govorunova; S Rank; P Hegemann
Journal:  J Cell Sci       Date:  2001-11       Impact factor: 5.285
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  9 in total

1.  An Sfi1p-like centrin-binding protein mediates centrin-based Ca2+ -dependent contractility in Paramecium tetraurelia.

Authors:  Delphine Gogendeau; Janine Beisson; Nicole Garreau de Loubresse; Jean-Pierre Le Caer; Françoise Ruiz; Jean Cohen; Linda Sperling; France Koll; Catherine Klotz
Journal:  Eukaryot Cell       Date:  2007-08-03

Review 2.  RNA silencing in Chlamydomonas: mechanisms and tools.

Authors:  Michael Schroda
Journal:  Curr Genet       Date:  2005-11-25       Impact factor: 3.886

3.  An inducible artificial microRNA system for Chlamydomonas reinhardtii confirms a key role for heat shock factor 1 in regulating thermotolerance.

Authors:  Stefan Schmollinger; Daniela Strenkert; Michael Schroda
Journal:  Curr Genet       Date:  2010-05-07       Impact factor: 3.886

Review 4.  RNA-mediated silencing in Algae: biological roles and tools for analysis of gene function.

Authors:  Heriberto Cerutti; Xinrong Ma; Joseph Msanne; Timothy Repas
Journal:  Eukaryot Cell       Date:  2011-07-29

5.  Sulphur responsiveness of the Chlamydomonas reinhardtii LHCBM9 promoter.

Authors:  Anne L Sawyer; Ben D Hankamer; Ian L Ross
Journal:  Planta       Date:  2015-02-12       Impact factor: 4.116

6.  Unstable RNAi effects through epigenetic silencing of an inverted repeat transgene in Chlamydomonas reinhardtii.

Authors:  Tomohito Yamasaki; Hitoshi Miyasaka; Takeshi Ohama
Journal:  Genetics       Date:  2008-10-01       Impact factor: 4.562

7.  Unrestrained markerless trait stacking in Nannochloropsis gaditana through combined genome editing and marker recycling technologies.

Authors:  John Verruto; Kristie Francis; Yingjun Wang; Melisa C Low; Jessica Greiner; Sarah Tacke; Fedor Kuzminov; William Lambert; Jay McCarren; Imad Ajjawi; Nicholas Bauman; Ryan Kalb; Gregory Hannum; Eric R Moellering
Journal:  Proc Natl Acad Sci U S A       Date:  2018-07-09       Impact factor: 11.205

Review 8.  Transgene Expression in Microalgae-From Tools to Applications.

Authors:  Lior Doron; Na'ama Segal; Michal Shapira
Journal:  Front Plant Sci       Date:  2016-04-22       Impact factor: 5.753

Review 9.  Good News for Nuclear Transgene Expression in Chlamydomonas.

Authors:  Michael Schroda
Journal:  Cells       Date:  2019-11-28       Impact factor: 6.600
  9 in total

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