| Literature DB >> 16278432 |
Li Xu1, Vicki Ensor1, Savita Gossain1, Kathy Nye1, Peter Hawkey1.
Abstract
A novel multiplex PCR assay is described (CTX-Mplex PCR) that allows rapid detection of bla(CTX-M) genes and discrimination between groups 1, 2, 9 and 25/26. The specificity and sensitivity of the assay were evaluated with 10 control strains and then applied to 62 clinical isolates. The multiplex PCR detected and classified bla(CTX-M) genes with 100 % accuracy. The utilization of a denaturing HPLC WAVE system to size the PCR products automatically from the multiplex PCR enhances the assay by saving time and costs.Mesh：
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Year: 2005 PMID： 16278432 DOI： 10.1099/jmm.0.46160-0
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472