INTRODUCTION: Helicobacter pylori infection is associated with the development of several gastroduodenal diseases. Bacterial virulence genes have been found associated with an increased risk for gastric disease. OBJECTIVES: Herein, associations were made between the presence of vacA, cagA, cagE, babA2 and oipA genes in H. pylori isolates and the range of clinical consequences of the infection. METHODS: PCR was used to amplify vacA, cagA, cagE, babA2 and oipA genes in 166 isolates-50 patients with peptic ulcer, 39 with non-atrophic gastritis, 26 with atrophic gastritis, 26 with intestinal metaplasia and 25 with gastric adenocarcinoma. RESULTS: cagA, cagE, babA2 and oipA genes were found in 73%, 75%, 48% and 74% of isolates, respectively. The cytotoxic vacA s1m1/cagA positive/cage positive genotype was present in 64% (100/157) of isolates. A higher frequency of cytotoxic strains was observed in cancer patients (84%), intestinal metaplasia (91%) and peptic ulcer (81%) in comparison with gastritis patients (50%) (p=0.002, 0.008, 0.007, respectively). The oipA and babA2 frequency was higher in cytotoxic isolates than in non-cytotoxic isolates (oipA: 81% vs. 52%, P=0,003; babA2: 58% vs. 12% (p<0.001). No significant association was found among clinical outcomes and oipA or babA2 genotypes, analyzed alone or in combination with vacA and cagA. CONCLUSION: Therefore, babA2 or oipA genes are not marker indicators of ulcer or cancer.
INTRODUCTION:Helicobacter pyloriinfection is associated with the development of several gastroduodenal diseases. Bacterial virulence genes have been found associated with an increased risk for gastric disease. OBJECTIVES: Herein, associations were made between the presence of vacA, cagA, cagE, babA2 and oipA genes in H. pylori isolates and the range of clinical consequences of the infection. METHODS: PCR was used to amplify vacA, cagA, cagE, babA2 and oipA genes in 166 isolates-50 patients with peptic ulcer, 39 with non-atrophic gastritis, 26 with atrophic gastritis, 26 with intestinal metaplasia and 25 with gastric adenocarcinoma. RESULTS:cagA, cagE, babA2 and oipA genes were found in 73%, 75%, 48% and 74% of isolates, respectively. The cytotoxic vacA s1m1/cagA positive/cage positive genotype was present in 64% (100/157) of isolates. A higher frequency of cytotoxic strains was observed in cancerpatients (84%), intestinal metaplasia (91%) and peptic ulcer (81%) in comparison with gastritispatients (50%) (p=0.002, 0.008, 0.007, respectively). The oipA and babA2 frequency was higher in cytotoxic isolates than in non-cytotoxic isolates (oipA: 81% vs. 52%, P=0,003; babA2: 58% vs. 12% (p<0.001). No significant association was found among clinical outcomes and oipA or babA2 genotypes, analyzed alone or in combination with vacA and cagA. CONCLUSION: Therefore, babA2 or oipA genes are not marker indicators of ulcer or cancer.
Authors: Carlos Alberto Fajardo; Andrés Javier Quiroga; Andrea Coronado; Karen Labrador; Nicole Acosta; Pilar Delgado; Carlos Jaramillo; María Mercedes Bravo Journal: World J Gastrointest Oncol Date: 2013-03-15
Authors: M Constanza Camargo; M Blanca Piazuelo; Robertino M Mera; Elizabeth T H Fontham; Alberto G Delgado; M Clara Yepez; Cristina Ceron; Luis E Bravo; Juan C Bravo; Pelayo Correa Journal: Acta Gastroenterol Latinoam Date: 2007-12