Primary sequence of -1436 to +53 bp of the rat preproenkephalin gene putative Z-DNA and regulatory motifs.

We report novel sequence data extending -1436 bases 5' of the rat proenkephalin gene start site known as E4. We noted an interesting stretch of 58 bases of alternating pyrimidines that lies immediately adjacent to 71 bases of an alternating purine-pyrimidine Z-DNA-like sequence that lies between -694 bp and -566 bp. Multiple sequence homologies to putative cis-acting regulatory factor binding sites were identified by a computer aided sequence search.