Literature DB >> 1627648

PCR cloning and nucleotide sequence determination of the 18S rRNA genes and internal transcribed spacer 1 of the protozoan parasites Cryptosporidium parvum and Cryptosporidium muris.

J Cai1, M D Collins, V McDonald, D E Thompson.   

Abstract

The genes encoding 18S rRNA and internal transcribed spacer 1 (1TS1) of Cryptosporidium parvum and Cryptosporidium muris were amplified from oocysts by PCR utilizing primers complementary to conserved regions of the 5' end of 18S and 5.8S rRNA. PCR products were cloned and the complete nucleotide sequences of two clones of each Cryptosporidium species were determined. The 18S rRNA genes of C. parvum and C. muris showed more than 99% sequence identity.

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Year:  1992        PMID： 1627648     DOI： 10.1016/0167-4781(92)90032-u

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  15 in total

1.  Sources and species of cryptosporidium oocysts in the Wachusett Reservoir watershed.

Authors:  Kristen L Jellison; Harold F Hemond; David B Schauer
Journal:  Appl Environ Microbiol       Date:  2002-02       Impact factor: 4.792

2.  Evaluation of Cryptosporidium parvum genotyping techniques.

Authors:  I M Sulaiman; L Xiao; A A Lal
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

3.  Molecular analysis of the 18S rRNA gene of Cryptosporidium serpentis in a wild-caught corn snake (Elaphe guttata guttata) and a five-species restriction fragment length polymorphism- based assay that can additionally discern C. parvum from C. wrairi.

Authors:  L M Kimbell; D L Miller; W Chavez; N Altman
Journal:  Appl Environ Microbiol       Date:  1999-12       Impact factor: 4.792

4.  Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus.

Authors:  L Xiao; L Escalante; C Yang; I Sulaiman; A A Escalante; R J Montali; R Fayer; A A Lal
Journal:  Appl Environ Microbiol       Date:  1999-04       Impact factor: 4.792

5.  Characterization of Hepatozoon spp. in Leptodactylus chaquensis and Leptodactylus podicipinus from two regions of the Pantanal, state of Mato Grosso do Sul, Brazil.

Authors:  Denise Dutra Menezes Leal; Carine Spenassatto Dreyer; Reinaldo José da Silva; Paulo Eduardo Martins Ribolla; Karina dos Santos Paduan; Inácio Bianchi; Lucia Helena O'Dwyer
Journal:  Parasitol Res       Date:  2015-02-03       Impact factor: 2.289

6.  Intra-isolate variation of Cryptosporidium parvum small subunit ribosomal RNA genes from human hosts in England.

Authors:  Cindy Gibbons-Matthews; Annette M Prescott
Journal:  Parasitol Res       Date:  2003-05-23       Impact factor: 2.289

7.  Identification of Cryptosporidium isolates from cockatiels by direct sequencing of the PCR-amplified small subunit ribosomal RNA gene.

Authors:  Niichiro Abe; Motohiro Iseki
Journal:  Parasitol Res       Date:  2004-03-04       Impact factor: 2.289

8.  Cryptosporidium parvum induces an endoplasmic stress response in the intestinal adenocarcinoma HCT-8 cell line.

Authors:  Mary Morada; Lakhsmi Pendyala; Gang Wu; Salim Merali; Nigel Yarlett
Journal:  J Biol Chem       Date:  2013-08-28       Impact factor: 5.157

9.  Isoenzyme variation within the genus Cryptosporidium.

Authors:  B W Ogunkolade; H A Robinson; V McDonald; K Webster; D A Evans
Journal:  Parasitol Res       Date:  1993       Impact factor: 2.289

10.  Identification of genetic heterogeneity in the Cryptosporidium parvum ribosomal repeat.

Authors:  M Carraway; S Tzipori; G Widmer
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792
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