Literature DB >> 16275331

Enzymology of the anaphase-promoting complex.

Christopher W Carroll1, David O Morgan.   

Abstract

The anaphase-promoting complex (APC) is an ubiquitin-protein ligase that promotes mitotic progression by catalyzing the ubiquitination of numerous proteins, including securin and cyclin. Its complex subunit composition and extensive regulation make the APC an active subject of investigation for both cell biologists and enzymologists. This chapter describes a system for the reconstitution and quantitative analysis of APC activity from budding yeast in vitro. We focus in particular on the measurement of processive ubiquitination, which complements traditional analysis of the reaction rate as a means to elucidate the molecular details of substrate recognition and ubiquitination by the APC.

Entities:  

Mesh：

Substances：

Year:  2005        PMID： 16275331     DOI： 10.1016/S0076-6879(05)98018-X

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  22 in total

1.  Ubiquitination of Cdc20 by the APC occurs through an intramolecular mechanism.

Authors:  Ian T Foe; Scott A Foster; Stephanie K Cheung; Steven Z DeLuca; David O Morgan; David P Toczyski
Journal:  Curr Biol       Date:  2011-11-10       Impact factor: 10.834

2.  Protein-linked ubiquitin chain structure restricts activity of deubiquitinating enzymes.

Authors:  Jonathan B Schaefer; David O Morgan
Journal:  J Biol Chem       Date:  2011-11-09       Impact factor: 5.157

3.  An E2 accessory domain increases affinity for the anaphase-promoting complex and ensures E2 competition.

Authors:  Juliet R Girard; Jeanette L Tenthorey; David O Morgan
Journal:  J Biol Chem       Date:  2015-08-25       Impact factor: 5.157

4.  RNAi of mitotic cyclins in Drosophila uncouples the nuclear and centrosome cycle.

Authors:  Mark L McCleland; Patrick H O'Farrell
Journal:  Curr Biol       Date:  2008-02-26       Impact factor: 10.834

5.  Evolution of Ime2 phosphorylation sites on Cdk1 substrates provides a mechanism to limit the effects of the phosphatase Cdc14 in meiosis.

Authors:  Liam J Holt; Jessica E Hutti; Lewis C Cantley; David O Morgan
Journal:  Mol Cell       Date:  2007-03-09       Impact factor: 17.970

6.  Modulation of the mitotic regulatory network by APC-dependent destruction of the Cdh1 inhibitor Acm1.

Authors:  Maria Enquist-Newman; Matt Sullivan; David O Morgan
Journal:  Mol Cell       Date:  2008-05-23       Impact factor: 17.970

7.  The pseudosubstrate inhibitor Acm1 inhibits the anaphase-promoting complex/cyclosome by combining high-affinity activator binding with disruption of Doc1/Apc10 function.

Authors:  Liang Qin; Arda Mizrak; Dimitrius Santiago P S F Guimarães; Hana M Tambrin; David O Morgan; Mark C Hall
Journal:  J Biol Chem       Date:  2019-09-27       Impact factor: 5.157

8.  Functionally distinct isoforms of Cik1 are differentially regulated by APC/C-mediated proteolysis.

Authors:  Jennifer A Benanti; Mary E Matyskiela; David O Morgan; David P Toczyski
Journal:  Mol Cell       Date:  2009-03-13       Impact factor: 17.970

9.  An architectural map of the anaphase-promoting complex.

Authors:  Brian R Thornton; Tessie M Ng; Mary E Matyskiela; Christopher W Carroll; David O Morgan; David P Toczyski
Journal:  Genes Dev       Date:  2006-02-15       Impact factor: 11.361

10.  Unique D box and KEN box sequences limit ubiquitination of Acm1 and promote pseudosubstrate inhibition of the anaphase-promoting complex.

Authors:  Eunyoung Choi; J Michael Dial; Dah-Eun Jeong; Mark C Hall
Journal:  J Biol Chem       Date:  2008-07-02       Impact factor: 5.157
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.