Literature DB >> 16275328

Affinity purification of mitotic anaphase-promoting complex/cyclosome on p13Suc1.

Avram Hershko1.   

Abstract

A procedure is described for the affinity purification of the mitotic form of anaphase-promoting complex/cyclosome (APC/C) from HeLa cells. It is based on the binding of mitotically phosphorylated APC/C to the phosphate-binding site of p13(suc1), followed by specific elution with a phosphate-containing compound. The procedure is rapid, simple, and yields 50- to 70-fold purification of soluble APC/C, with a approximately 30% recovery of activity.

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Year:  2005        PMID： 16275328     DOI： 10.1016/S0076-6879(05)98015-4

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  4 in total

1.  Roles of the anaphase-promoting complex/cyclosome and of its activator Cdc20 in functional substrate binding.

Authors:  Esther Eytan; Yakir Moshe; Ilana Braunstein; Avram Hershko
Journal:  Proc Natl Acad Sci U S A       Date:  2006-02-02       Impact factor: 11.205

2.  Inhibitory factors associated with anaphase-promoting complex/cylosome in mitotic checkpoint.

Authors:  Ilana Braunstein; Shirly Miniowitz; Yakir Moshe; Avram Hershko
Journal:  Proc Natl Acad Sci U S A       Date:  2007-03-13       Impact factor: 11.205

3.  Regulation of the action of early mitotic inhibitor 1 on the anaphase-promoting complex/cyclosome by cyclin-dependent kinases.

Authors:  Yakir Moshe; Ortal Bar-On; Dvora Ganoth; Avram Hershko
Journal:  J Biol Chem       Date:  2011-03-16       Impact factor: 5.157

Review 4.  Processive ubiquitin chain formation by the anaphase-promoting complex.

Authors:  Hermann-Josef Meyer; Michael Rape
Journal:  Semin Cell Dev Biol       Date:  2011-04-06       Impact factor: 7.727
  4 in total

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