OBJECTIVE: To determine the concordance of day-6 blastocyst analysis with the day-3 fluorescence in situ hybridization (FISH) aneuploidy diagnosis. DESIGN: Retrospective study. SETTING: In vitro fertilization laboratory. PATIENT(S): Six hundred sixty embryos were included from 94 IVF/intracytoplasmic sperm injection patients undergoing preimplantation genetic diagnosis. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Single blastomeres biopsied on day 3. Aneuploidy screening for chromosomes 13, 18, 21, X, and Y were analyzed. Left-over blastocysts were reanalyzed on day 6. RESULT(S): Among the 660 embryos evaluated, 367 (55.6%) were euploid and 281 (42.6%) were aneuploid. Of the euploid embryos, 213 embryos were transferred, 68 were frozen on day 5, and 86 were left. All 281 aneuploid embryos were further cultured, and 55 (19.6%) progressed to blastocysts. When FISH reanalysis was performed, 33 of 55 blastocysts (60%) were confirmed aneuploid in concordance with the day-3 diagnosis. However, 22 of 55 blastocysts (40%) were determined to be euploid. In addition, 207 aneuploid embryos (73.7%) arrested before day 6, as opposed to 32 of the 86 euploid embryos (37.2%). CONCLUSION(S): Day-3 single-cell embryo biopsy reveals that aneuploidy can be confirmed in 60.7% of the blastocysts on reanalysis. The majority of discordance is most likely due to embryo mosaicism and possibly a limited ability to "self-correct."
OBJECTIVE: To determine the concordance of day-6 blastocyst analysis with the day-3 fluorescence in situ hybridization (FISH) aneuploidy diagnosis. DESIGN: Retrospective study. SETTING: In vitro fertilization laboratory. PATIENT(S): Six hundred sixty embryos were included from 94 IVF/intracytoplasmic sperm injection patients undergoing preimplantation genetic diagnosis. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Single blastomeres biopsied on day 3. Aneuploidy screening for chromosomes 13, 18, 21, X, and Y were analyzed. Left-over blastocysts were reanalyzed on day 6. RESULT(S): Among the 660 embryos evaluated, 367 (55.6%) were euploid and 281 (42.6%) were aneuploid. Of the euploid embryos, 213 embryos were transferred, 68 were frozen on day 5, and 86 were left. All 281 aneuploid embryos were further cultured, and 55 (19.6%) progressed to blastocysts. When FISH reanalysis was performed, 33 of 55 blastocysts (60%) were confirmed aneuploid in concordance with the day-3 diagnosis. However, 22 of 55 blastocysts (40%) were determined to be euploid. In addition, 207 aneuploid embryos (73.7%) arrested before day 6, as opposed to 32 of the 86 euploid embryos (37.2%). CONCLUSION(S): Day-3 single-cell embryo biopsy reveals that aneuploidy can be confirmed in 60.7% of the blastocysts on reanalysis. The majority of discordance is most likely due to embryo mosaicism and possibly a limited ability to "self-correct."
Authors: Chris M J van Uum; Servi J C Stevens; Joseph C F M Dreesen; Marion Drüsedau; Hubert J Smeets; Bertien Hollanders-Crombach; Christine E M de Die-Smulders; Joep P M Geraedts; John J M Engelen; Edith Coonen Journal: Eur J Hum Genet Date: 2012-02-29 Impact factor: 4.246
Authors: D Ioannou; K G L Fonseka; E J Meershoek; A R Thornhill; A Abogrein; M Ellis; D K Griffin Journal: Chromosome Res Date: 2012-06-29 Impact factor: 5.239
Authors: Miguel A Checa; Pablo Alonso-Coello; Ivan Solà; Ana Robles; Ramón Carreras; Juan Balasch Journal: J Assist Reprod Genet Date: 2009-07-24 Impact factor: 3.412
Authors: Nathan R Treff; Brynn Levy; Jing Su; Lesley E Northrop; Xin Tao; Richard T Scott Journal: Mol Hum Reprod Date: 2010-05-19 Impact factor: 4.025