| Literature DB >> 16274991 |
Christine Kurata1, Kym Bradley, Hans Gaus, Nhuy Luu, Isaiah Cedillo, Vasulinga T Ravikumar, Kent Van Sooy, James V McArdle, Daniel C Capaldi.
Abstract
Phosphorothioate oligonucleotides manufactured by standard phosphoramidite techniques using 2'-deoxyadenosine- or 2'-O-(2-methoxyethyl)-5-methylcytosine-loaded solid supports contain branched impurities consisting of two chains linked through the exocyclic amino group of the 3'-terminal nucleoside of one chain and the 3'-terminal hydroxyl group of another via a P(O)SH group. These impurities are not produced when a universal, non-nucleoside derivatized support is used.Entities:
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Year: 2005 PMID： 16274991 DOI： 10.1016/j.bmcl.2005.10.051
Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823