Literature DB >> 16269260

Hadamard frequency-encoded SOFAST-HMQC for ultrafast two-dimensional protein NMR.

Paul Schanda1, Bernhard Brutscher.   

Abstract

We demonstrate the feasibility of recording (1)H-(15)N correlation spectra of proteins in only one second of acquisition time. The experiment combines recently proposed SOFAST-HMQC with Hadamard-type (15)N frequency encoding. This allows site-resolved real-time NMR studies of kinetic processes in proteins with an increased time resolution. The sensitivity of the experiment is sufficient to be applicable to a wide range of molecular systems available at millimolar concentration on a high magnetic field spectrometer.

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Year:  2005        PMID： 16269260     DOI： 10.1016/j.jmr.2005.10.007

Source DB:  PubMed          Journal:  J Magn Reson        ISSN： 1090-7807            Impact factor:   2.229


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