| Literature DB >> 16261515 |
Omar Estrada1, Masahisa Hasegawa, Freddy Gonzalez-Mujíca, Norma Motta, Elsa Perdomo, Alejandra Solorzano, Jeannette Méndez, Bernardo Méndez, E Gabriela Zea.
Abstract
From the methanol extract of Bauhinia megalandra fresh leaves, eight flavonoids were isolated and evaluated by rat liver microsomal glucose-6-phosphatase (G-6-Pase) bioassay, which might be a useful methodology for screening antihyperglycaemic substances. All the flavonoids assayed showed an inhibitory effect on the intact microsomal G-6-Pase: quercetin and kaempferol exhibited the lowest effect; astilbin, quercetin 3-O-alpha-rhamnoside, kaempferol 3-O-alpha-rhamnoside and quercetin 3-O-alpha-arabinoside an intermediate effect. The highest inhibitory activity was shown by quercetin 3-O-alpha-(2''-galloyl)rhamnoside and kaempferol 3-O-alpha-(2''galloyl)rhamnoside. None of the flavonoids mentioned above showed an inhibitory effect on the disrupted microsomal G-6-Pase. Quercetin 3-O-alpha-(2''-galloyl)rhamnoside and kaempferol 3-O-alpha-(2''-galloyl)rhamnoside exhibited the lowest IC50 of all the flavonoids assayed. Also, the phlorizin IC50 is reported. Copyright (c) 2005 John Wiley & Sons, Ltd.Entities:
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Year: 2005 PMID： 16261515 DOI： 10.1002/ptr.1703
Source DB: PubMed Journal: Phytother Res ISSN： 0951-418X Impact factor: 5.878