PURPOSE: To analyze the effect of 3 months of training on global gene expression in skeletal muscle of healthy elderly men in order to better characterize the pathways that differentiate the trained from the sedentary state. METHODS: Needle biopsies were obtained from the vastus lateralis of six healthy, sedentary, 68.0 +/- 2.7-yr-old males, before and after 3 months of training at 80% of maximal capacity, that improved peak O2 uptake by 17.8 +/- 4.0% (P = 0.009). RNA extracted from the samples was hybridized to Affymetrix U133A Genechip arrays. RESULTS: Overall, training modified the expression of 397 out of 14,500 genes tested. In the trained state, the expression of genes linked with energy metabolism, protein amino acid dephosphorylation, and heme biosynthesis increased, whereas those linked with ribosome and protein catabolism decreased. CONCLUSION: This set of 397 genes that includes the upregulated energy pathways and the downregulated protein catabolism genes represents the transcriptional response of the skeletal muscle in the trained state. It is highly likely that many of these genes are mediators of the beneficial effects of physical activity on health and fitness.
PURPOSE: To analyze the effect of 3 months of training on global gene expression in skeletal muscle of healthy elderly men in order to better characterize the pathways that differentiate the trained from the sedentary state. METHODS: Needle biopsies were obtained from the vastus lateralis of six healthy, sedentary, 68.0 +/- 2.7-yr-old males, before and after 3 months of training at 80% of maximal capacity, that improved peak O2 uptake by 17.8 +/- 4.0% (P = 0.009). RNA extracted from the samples was hybridized to Affymetrix U133A Genechip arrays. RESULTS: Overall, training modified the expression of 397 out of 14,500 genes tested. In the trained state, the expression of genes linked with energy metabolism, protein amino acid dephosphorylation, and heme biosynthesis increased, whereas those linked with ribosome and protein catabolism decreased. CONCLUSION: This set of 397 genes that includes the upregulated energy pathways and the downregulated protein catabolism genes represents the transcriptional response of the skeletal muscle in the trained state. It is highly likely that many of these genes are mediators of the beneficial effects of physical activity on health and fitness.
Authors: Timothy W Rhoads; Josef P Clark; Grace E Gustafson; Karl N Miller; Matthew W Conklin; Tyler M DeMuth; Mark E Berres; Kevin W Eliceiri; Laura K Vaughan; Christine W Lary; T Mark Beasley; Ricki J Colman; Rozalyn M Anderson Journal: Cell Syst Date: 2020-01-22 Impact factor: 10.304
Authors: James A Timmons; Steen Knudsen; Tuomo Rankinen; Lauren G Koch; Mark Sarzynski; Thomas Jensen; Pernille Keller; Camilla Scheele; Niels B J Vollaard; Søren Nielsen; Thorbjörn Akerström; Ormond A MacDougald; Eva Jansson; Paul L Greenhaff; Mark A Tarnopolsky; Luc J C van Loon; Bente K Pedersen; Carl Johan Sundberg; Claes Wahlestedt; Steven L Britton; Claude Bouchard Journal: J Appl Physiol (1985) Date: 2010-02-04
Authors: Nicolas J Pillon; Brendan M Gabriel; Lucile Dollet; Jonathon A B Smith; Laura Sardón Puig; Javier Botella; David J Bishop; Anna Krook; Juleen R Zierath Journal: Nat Commun Date: 2020-01-24 Impact factor: 14.919
Authors: Moisés Selman; Guillermo Carrillo; Andrea Estrada; Mayra Mejia; Carina Becerril; José Cisneros; Miguel Gaxiola; Rogelio Pérez-Padilla; Carmen Navarro; Thomas Richards; James Dauber; Talmadge E King; Annie Pardo; Naftali Kaminski Journal: PLoS One Date: 2007-05-30 Impact factor: 3.240
Authors: Pénélope A Andreux; Marcus P J van Diemen; Maxime R Heezen; Johan Auwerx; Chris Rinsch; Geert Jan Groeneveld; Anurag Singh Journal: Sci Rep Date: 2018-06-04 Impact factor: 4.379