Literature DB >> 16260152

Specific detection of RNA on ultra-thin sections.

Juana Calabuig1, Joaquin de Juan, Francisco J Iborra.   

Abstract

In this paper, we describe a specific method for ultrastructural detection of RNA. Our method is based in the bromination "in situ" of uridine residues in the RNA, which allows the detection of brominated RNA with specific antibodies against bromo-deoxyuridine. With this method we can achieve high specificity and resolution, and it can be applied to Epon or acrylic resin embedded material.

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Year:  2005        PMID: 16260152     DOI: 10.1016/j.jsb.2005.06.005

Source DB:  PubMed          Journal:  J Struct Biol        ISSN: 1047-8477            Impact factor:   2.867


  2 in total

1.  Wide confocal cytometry: a new approach to study proteomic and structural changes in the cell nucleus during the cell cycle.

Authors:  Francisco J Iborra; Veronica Buckle
Journal:  Histochem Cell Biol       Date:  2007-11-08       Impact factor: 4.304

2.  Global variability in gene expression and alternative splicing is modulated by mitochondrial content.

Authors:  Raul Guantes; Alberto Rastrojo; Ricardo Neves; Ana Lima; Begoña Aguado; Francisco J Iborra
Journal:  Genome Res       Date:  2015-03-23       Impact factor: 9.043

  2 in total

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