Literature DB >> 16254049

Arp2/3 complex-deficient mouse fibroblasts are viable and have normal leading-edge actin structure and function.

Alessia Di Nardo1, Gregor Cicchetti, Hervé Falet, John H Hartwig, Thomas P Stossel, David J Kwiatkowski.   

Abstract

RNA interference silencing of up to 90% of Arp3 protein expression, a major subunit of the Arp2/3 complex, proportionately decreases the intracellular motility of Listeria monocytogenes and actin nucleation activity ascribable to the Arp2/3 complex in mouse embryonic fibroblasts. However, the Arp2/3-deficient cells exhibit unimpaired lamellipodial actin network structure, translational locomotion, spreading, actin assembly, and ruffling responses. In addition, Arp3-silenced cells expressing neural Wiskott-Aldrich syndrome protein-derived peptides that inhibit Arp2/3 complex function in wild-type cells retained normal PDGF-induced ruffling. The Arp2/3 complex can be dispensable for leading-edge actin remodeling.

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Year:  2005        PMID： 16254049      PMCID： PMC1283463          DOI： 10.1073/pnas.0508228102

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  31 in total

1.  Mechanisms of cold-induced platelet actin assembly.

Authors:  K M Hoffmeister; H Falet; A Toker; K L Barkalow; T P Stossel; J H Hartwig
Journal:  J Biol Chem       Date:  2001-04-27       Impact factor: 5.157

2.  Involvement of the Arp2/3 complex in phagocytosis mediated by FcgammaR or CR3.

Authors:  R C May; E Caron; A Hall; L M Machesky
Journal:  Nat Cell Biol       Date:  2000-04       Impact factor: 28.824

3.  Reconstitution of human Arp2/3 complex reveals critical roles of individual subunits in complex structure and activity.

Authors:  H Gournier; E D Goley; H Niederstrasser; T Trinh; M D Welch
Journal:  Mol Cell       Date:  2001-11       Impact factor: 17.970

4.  Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.

Authors:  Patrick J Paddison; Amy A Caudy; Emily Bernstein; Gregory J Hannon; Douglas S Conklin
Journal:  Genes Dev       Date:  2002-04-15       Impact factor: 11.361

Review 5.  The lamellipodium: where motility begins.

Authors:  J Victor Small; Theresia Stradal; Emmanuel Vignal; Klemens Rottner
Journal:  Trends Cell Biol       Date:  2002-03       Impact factor: 20.808

6.  Comparison of filamin A-induced cross-linking and Arp2/3 complex-mediated branching on the mechanics of actin filaments.

Authors:  Fumihiko Nakamura; Eric Osborn; Paul A Janmey; Thomas P Stossel
Journal:  J Biol Chem       Date:  2002-01-10       Impact factor: 5.157

7.  Identification of essential genes in cultured mammalian cells using small interfering RNAs.

Authors:  J Harborth; S M Elbashir; K Bechert; T Tuschl; K Weber
Journal:  J Cell Sci       Date:  2001-12       Impact factor: 5.285

8.  Filamin A, the Arp2/3 complex, and the morphology and function of cortical actin filaments in human melanoma cells.

Authors:  L A Flanagan; J Chou; H Falet; R Neujahr; J H Hartwig; T P Stossel
Journal:  J Cell Biol       Date:  2001-11-12       Impact factor: 10.539

9.  Cell migration without a lamellipodium: translation of actin dynamics into cell movement mediated by tropomyosin.

Authors:  Stephanie L Gupton; Karen L Anderson; Thomas P Kole; Robert S Fischer; Aaron Ponti; Sarah E Hitchcock-DeGregori; Gaudenz Danuser; Velia M Fowler; Denis Wirtz; Dorit Hanein; Clare M Waterman-Storer
Journal:  J Cell Biol       Date:  2005-02-14       Impact factor: 10.539

10.  A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex.

Authors:  Andrew M Hudson; Lynn Cooley
Journal:  J Cell Biol       Date:  2002-02-18       Impact factor: 10.539
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  29 in total

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Journal:  Mol Biol Cell       Date:  2008-02-06       Impact factor: 4.138

2.  Organization of the Arp2/3 complex in hippocampal spines.

Authors:  Bence Rácz; Richard J Weinberg
Journal:  J Neurosci       Date:  2008-05-28       Impact factor: 6.167

3.  The role of filament-packing dynamics in powering amoeboid cell motility.

Authors:  Long Miao; Orion Vanderlinde; Jun Liu; Richard P Grant; Alan Wouterse; Katsuya Shimabukuro; Albert Philipse; Murray Stewart; Thomas M Roberts
Journal:  Proc Natl Acad Sci U S A       Date:  2008-04-02       Impact factor: 11.205

4.  The role of Arp2/3 in growth cone actin dynamics and guidance is substrate dependent.

Authors:  José E San Miguel-Ruiz; Paul C Letourneau
Journal:  J Neurosci       Date:  2014-04-23       Impact factor: 6.167

5.  FHOD1 is needed for directed forces and adhesion maturation during cell spreading and migration.

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Journal:  Dev Cell       Date:  2013-12-09       Impact factor: 12.270

6.  Helicobacter pylori CagA induces AGS cell elongation through a cell retraction defect that is independent of Cdc42, Rac1, and Arp2/3.

Authors:  Kevin M Bourzac; Crystal M Botham; Karen Guillemin
Journal:  Infect Immun       Date:  2006-12-28       Impact factor: 3.441

Review 7.  New insights into the formation and the function of lamellipodia and ruffles in mesenchymal cell migration.

Authors:  Metello Innocenti
Journal:  Cell Adh Migr       Date:  2018-05-08       Impact factor: 3.405

8.  Nano-scale actin-network characterization of fibroblast cells lacking functional Arp2/3 complex.

Authors:  Karen L Anderson; Christopher Page; Mark F Swift; Praveen Suraneni; Mandy E W Janssen; Thomas D Pollard; Rong Li; Niels Volkmann; Dorit Hanein
Journal:  J Struct Biol       Date:  2016-12-21       Impact factor: 2.867

9.  Gadkin negatively regulates cell spreading and motility via sequestration of the actin-nucleating ARP2/3 complex.

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Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-11       Impact factor: 11.205

Review 10.  Self-organization of protrusions and polarity during eukaryotic chemotaxis.

Authors:  Brian R Graziano; Orion D Weiner
Journal:  Curr Opin Cell Biol       Date:  2014-07-05       Impact factor: 8.382
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