OBJECTIVE: To observe the effect of volatile oil and aqueous soluable part of Piper longum on proliferation of rat mesenchymal stem cell (MSC) and the chemical functional groups. METHODS: Mesenchymal stem cells were dissociated from rat bone marrow and marked by Brdu, and the expression of CD44, CD54 and double label of Brdu and CD44. The growth of rat mesenchymal stem cell under volatile oil and aqueous soluable part of Piper longum was observed by means of cell viability measurement (MTT) and morphological observation and Brdu, PCNA immunohistochemical methods. RESULTS: Volatile oil of Piper longum could promote the cell viability of MSC and the number of Brdu, PCNA positive cell in dose-dependant. There was significant difference in comparision with control groups, C = C(50.66%), -OH(27.02%) and other functional groups in volatile oil of Piper longum were determined by GC-MS, but the aqueous soluable part of Piper longum could not promote the proliferation of MSC. CONCLUSIONS: Volatile oil of Piper longum which consists of C = C, -OH, and other functional groups has strong effect on enhancing proliferation of MSC.
OBJECTIVE: To observe the effect of volatile oil and aqueous soluable part of Piper longum on proliferation of rat mesenchymal stem cell (MSC) and the chemical functional groups. METHODS: Mesenchymal stem cells were dissociated from rat bone marrow and marked by Brdu, and the expression of CD44, CD54 and double label of Brdu and CD44. The growth of rat mesenchymal stem cell under volatile oil and aqueous soluable part of Piper longum was observed by means of cell viability measurement (MTT) and morphological observation and Brdu, PCNA immunohistochemical methods. RESULTS:Volatile oil of Piper longum could promote the cell viability of MSC and the number of Brdu, PCNA positive cell in dose-dependant. There was significant difference in comparision with control groups, C = C(50.66%), -OH(27.02%) and other functional groups in volatile oil of Piper longum were determined by GC-MS, but the aqueous soluable part of Piper longum could not promote the proliferation of MSC. CONCLUSIONS:Volatile oil of Piper longum which consists of C = C, -OH, and other functional groups has strong effect on enhancing proliferation of MSC.