Literature DB >> 16252134

Expression of the Bacillus thuringiensis mosquitocidal toxin Cry11Aa in the aquatic bacterium Asticcacaulis excentricus.

Gemma Armengol1, Oscar Enrique Guevara, Sergio Orduz, Neil Crickmore.   

Abstract

A mosquitocidal aquatic bacterium has been developed by introducing an operon containing the cry11Aa, and p20 genes from Bacillus thuringiensis subsp. israelensis (Bti) into the gram-negative aquatic bacterium Asticcacaulis excentricus. After transformation, the cry11Aa gene was successfully expressed in recombinant A. excentricus under the tac promoter, at the level of 0.04 pg/cell. The recombinant bacteria were toxic to Aedes aegypti larvae with an LC(50) of 6.83 x 10(5) cells/mL. We believe that these bacteria may have potential as genetically engineered microorganisms for the control of mosquito larvae.

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Year:  2005        PMID： 16252134     DOI： 10.1007/s00284-005-0166-z

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  14 in total

1.  Creatures of our own making.

Authors:  Stephen Budiansky
Journal:  Science       Date:  2002-10-04       Impact factor: 47.728

2.  Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae.

Authors:  T Thanabalu; J Hindley; S Brenner; C Oei; C Berry
Journal:  Appl Environ Microbiol       Date:  1992-03       Impact factor: 4.792

3.  Development of a method for heterologous gene expression in Enterobacter amnigenus, a potential host for the biological control of mosquito larvi.

Authors:  Nawarat Nantapong; Sutipa Tanapongpipat; Jeffrey Cole; Sakol Panyim
Journal:  J Microbiol Methods       Date:  2002-05       Impact factor: 2.363

4.  Efficient expression of mosquito-larvicidal proteins in a gram-negative bacterium capable of recolonization in the guts of Anopheles dirus larva.

Authors:  P Khampang; W Chungjatupornchai; P Luxananil; S Panyim
Journal:  Appl Microbiol Biotechnol       Date:  1999-01       Impact factor: 4.813

5.  Expression of mosquito active toxin genes by a Colombian native strain of the gram-negative bacterium Asticcacaulis excentricus.

Authors:  M Romero; F M Gil; S Orduz
Journal:  Mem Inst Oswaldo Cruz       Date:  2001-02       Impact factor: 2.743

6.  Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis.

Authors:  E Ben-Dov; S Boussiba; A Zaritsky
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

7.  Influence of transcriptional and translational control sequences on the expression of foreign genes in Caulobacter crescentus.

Authors:  W H Yap; T Thanabalu; A G Porter
Journal:  J Bacteriol       Date:  1994-05       Impact factor: 3.490

8.  Expression of mosquitocidal toxin genes in a gas-vacuolated strain of Ancylobacter aquaticus.

Authors:  W H Yap; T Thanabalu; A G Porter
Journal:  Appl Environ Microbiol       Date:  1994-11       Impact factor: 4.792

9.  Improved production of the insecticidal CryIVD protein in Bacillus thuringiensis using cryIA(c) promoters to express the gene for an associated 20-kDa protein.

Authors:  D Wu; B A Federici
Journal:  Appl Microbiol Biotechnol       Date:  1995-01       Impact factor: 4.813

10.  Efficient synthesis of mosquitocidal toxins in Asticcacaulis excentricus demonstrates potential of gram-negative bacteria in mosquito control.

Authors:  J W Liu; W H Yap; T Thanabalu; A G Porter
Journal:  Nat Biotechnol       Date:  1996-03       Impact factor: 54.908
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  4 in total

1.  Large crystal toxin formation in chromosomally engineered Bacillus thuringiensis subsp. aizawai due to σE accumulation.

Authors:  Wasin Buasri; Watanalai Panbangred
Journal:  Appl Environ Microbiol       Date:  2012-01-20       Impact factor: 4.792

2.  Co-expression of the mosquitocidal toxins Cyt1Aa and Cry11Aa from Bacillus thuringiensis subsp. israelensis in Asticcacaulis excentricus.

Authors:  Dasheng Zheng; Norma Adriana Valdez-Cruz; Gemma Armengol; Chloe Sevrez; Jose Maurilio Munoz-Olaya; Zhiming Yuan; Sergio Orduz; Neil Crickmore
Journal:  Curr Microbiol       Date:  2006-12-06       Impact factor: 2.188

3.  Production of an insecticidal crystal protein from Bacillus thuringiensis by the methylotroph Methylobacterium extorquens.

Authors:  Young J Choi; J Lawrence Gringorten; Louise Bélanger; Lyne Morel; Denis Bourque; Luke Masson; Denis Groleau; Carlos B Míguez
Journal:  Appl Environ Microbiol       Date:  2008-06-13       Impact factor: 4.792

4.  The susceptibility of five African Anopheles species to Anabaena PCC 7120 expressing Bacillus thuringiensis subsp. israelensis mosquitocidal cry genes.

Authors:  Irene Ketseoglou; Gustav Bouwer
Journal:  Parasit Vectors       Date:  2012-10-04       Impact factor: 3.876
  4 in total

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