Literature DB >> 16252132

Purification and characterization of Cry1Ac toxin binding proteins from the brush border membrane of Helicoverpa armigera midgut.

Chunyan Liao1, Stephen C Trowell, Ray Akhurst.   

Abstract

Several Cry1Ac binding proteins from midgut of Helicoverpa armigera were purified using toxin-affinity chromatography. Enzyme assays showed that the purified proteins had strong aminopeptidase activity. The N-terminal sequences confidently identified a 124-kDa binding protein as an aminopeptidase N (APN), and some similarity suggests that a 162-kDa binding protein may also be an APN. Two minor binding proteins were not characterized.

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Year:  2005        PMID： 16252132     DOI： 10.1007/s00284-005-0051-9

Source DB:  PubMed          Journal:  Curr Microbiol        ISSN： 0343-8651            Impact factor:   2.188


  25 in total

1.  The quantitative histochemistry of brain. II. Enzyme measurements.

Authors:  O H LOWRY; N R ROBERTS; M L WU; W S HIXON; E J CRAWFORD
Journal:  J Biol Chem       Date:  1954-03       Impact factor: 5.157

2.  Isolation and partial characterization of gypsy moth BTR-270, an anionic brush border membrane glycoconjugate that binds Bacillus thuringiensis Cry1A toxins with high affinity.

Authors:  A P Valaitis; J L Jenkins; M K Lee; D H Dean; K J Garner
Journal:  Arch Insect Biochem Physiol       Date:  2001-04       Impact factor: 1.698

3.  Partial purification and characterization of Bacillus thuringiensis Cry1A toxin receptor A from Heliothis virescens and cloning of the corresponding cDNA.

Authors:  D I Oltean; A K Pullikuth; H K Lee; S S Gill
Journal:  Appl Environ Microbiol       Date:  1999-11       Impact factor: 4.792

4.  Transgenic Drosophila reveals a functional in vivo receptor for the Bacillus thuringiensis toxin Cry1Ac1.

Authors:  Michael Gill; David Ellar
Journal:  Insect Mol Biol       Date:  2002-12       Impact factor: 3.585

5.  Identification of a gene associated with Bt resistance in Heliothis virescens.

Authors:  L J Gahan; F Gould; D G Heckel
Journal:  Science       Date:  2001-08-03       Impact factor: 47.728

6.  The heliothis virescens 170 kDa aminopeptidase functions as "receptor A" by mediating specific Bacillus thuringiensis Cry1A delta-endotoxin binding and pore formation.

Authors:  K Luo; S Sangadala; L Masson; A Mazza; R Brousseau; M J Adang
Journal:  Insect Biochem Mol Biol       Date:  1997 Aug-Sep       Impact factor: 4.714

Review 7.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

8.  Insect resistant cotton plants.

Authors:  F J Perlak; R W Deaton; T A Armstrong; R L Fuchs; S R Sims; J T Greenplate; D A Fischhoff
Journal:  Biotechnology (N Y)       Date:  1990-10

9.  Identification of putative insect brush border membrane-binding molecules specific to Bacillus thuringiensis delta-endotoxin by protein blot analysis.

Authors:  S F Garczynski; J W Crim; M J Adang
Journal:  Appl Environ Microbiol       Date:  1991-10       Impact factor: 4.792

10.  The receptor for Bacillus thuringiensis CrylA(c) delta-endotoxin in the brush border membrane of the lepidopteran Manduca sexta is aminopeptidase N.

Authors:  P J Knight; N Crickmore; D J Ellar
Journal:  Mol Microbiol       Date:  1994-02       Impact factor: 3.501
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  4 in total

1.  Expression of recombinant and mosaic Cry1Ac receptors from Helicoverpa armigera and their influences on the cytotoxicity of activated Cry1Ac to Spodoptera litura Sl-HP cells.

Authors:  Peng Xu; Mayira Islam; Yutao Xiao; Fei He; Yi Li; Jianxin Peng; Huazhu Hong; Chenxi Liu; Kaiyu Liu
Journal:  Cytotechnology       Date:  2014-11-21       Impact factor: 2.058

2.  Analysis of Cry1Ab toxin bioaccumulation in a food chain of Bt rice, an herbivore and a predator.

Authors:  Mao Chen; Gong-yin Ye; Zhi-cheng Liu; Qi Fang; Cui Hu; Yu-fa Peng; Anthony M Shelton
Journal:  Ecotoxicology       Date:  2008-11-04       Impact factor: 2.823

3.  Diversity of aminopeptidases, derived from four lepidopteran gene duplications, and polycalins expressed in the midgut of Helicoverpa armigera: identification of proteins binding the delta-endotoxin, Cry1Ac of Bacillus thuringiensis.

Authors:  Constanza Angelucci; Gregory A Barrett-Wilt; Donald F Hunt; Raymond J Akhurst; Peter D East; Karl H J Gordon; Peter M Campbell
Journal:  Insect Biochem Mol Biol       Date:  2008-04-04       Impact factor: 4.714

4.  Exploring the midgut transcriptome and brush border membrane vesicle proteome of the rice stem borer, Chilo suppressalis (Walker).

Authors:  Weihua Ma; Zan Zhang; Chuanhua Peng; Xiaoping Wang; Fei Li; Yongjun Lin
Journal:  PLoS One       Date:  2012-05-29       Impact factor: 3.240
  4 in total

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