[Determination of relative molecular mass and composition for Polygonatum sibiricum polysaccharide by high performance liquid chromatography].

Polygonatum sibiricum Redoute (PSR) is a traditional Chinese medicine and Mongolia herbal medicine growing in Inner Mongolia. Polygonatum Sibiricum Polysaccharide (PSP) was isolated and purified with boiling water extraction, alcohol precipitation from the wild species roots of PSR. Proteins was removed from the crude PSP by methods of trypsin hydrolysate and Sevag. Purity, relative molecular mass and relative molecular mass distribution of PSP were determined using high performance liquid chromatography (HPLC). The buffer solution of NaH2PO4-Na2HPO4 (0.02 mol/L) was used as mobile phase, and the PSP was separated on a Shodex GPC column as a single symmetrical peak corresponding to a number mean molecular mass (Mn) of 7073 and relative molecular mass (Mr) of 7247, and polydispersity is 1.025. The sugar components of the PSP was analysed by HPLC and 13C NMR. Using water as mobile phase, the components of all acid hydrolysis of the PSP were separated on a Shodex SP0810 column. Glucose, galactose, mannose and fructose were used as the standard samples. The result showed that PSP is composed of fructose only.