| Literature DB >> 16246331 |
Maria Gómez-Benito1, Patricia Balsas, Alberto Bosque, Alberto Anel, Isabel Marzo, Javier Naval.
Abstract
Interferon-alpha (IFN-alpha) is currently used for the therapy of multiple myeloma (MM) though it is only effective in some patients. IFN-alpha induces apoptosis in some MM cell lines and it has been proposed to occur through an autocrine loop involving Apo2L/TRAIL. We have analysed the sensitivity to IFN-alpha and Apo2L/TRAIL of five MM cell lines and found no correlation between the apoptosis inducing ability of both cytokines. IFN-alpha-induced apoptosis in MM cells was not prevented by a caspase-8 selective inhibitor (Z-IETD-fmk) or blocking Apo2L/TRAIL. However, human monocytes treated with IFN-alpha release bioactive Apo2L/TRAIL to culture media which was cytotoxic for MM cells resistant to IFN-alpha. We propose that Apo2L/TRAIL released from IFN-alpha-stimulated blood monocytes would be a major mediator of the anti-myeloma effect of IFN-alpha in vivo.Entities:
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Year: 2005 PMID： 16246331 DOI： 10.1016/j.febslet.2005.10.007
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124