Literature DB >> 16245046

Characterization by Mass Spectroscopy of a 10 kDa c-554 Cytochrome from the Green Sulfur Bacterium Chlorobium tepidum.

Hervé-W Rémigy1, Michalis Aivaliotis, Nikos Ioannidis, Paul Jenö, Thierry Mini, Andreas Engel, Michel Jaquinod, Georgios Tsiotis.   

Abstract

Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.

Entities:  

Year:  2003        PMID： 16245046     DOI： 10.1023/B:PRES.0000004347.34228.2c

Source DB:  PubMed          Journal:  Photosynth Res        ISSN： 0166-8595            Impact factor:   3.429


  17 in total

1.  The complete genome sequence of Chlorobium tepidum TLS, a photosynthetic, anaerobic, green-sulfur bacterium.

Authors:  Jonathan A Eisen; Karen E Nelson; Ian T Paulsen; John F Heidelberg; Martin Wu; Robert J Dodson; Robert Deboy; Michelle L Gwinn; William C Nelson; Daniel H Haft; Erin K Hickey; Jeremy D Peterson; A Scott Durkin; James L Kolonay; Fan Yang; Ingeborg Holt; Lowell A Umayam; Tanya Mason; Michael Brenner; Terrance P Shea; Debbie Parksey; William C Nierman; Tamara V Feldblyum; Cheryl L Hansen; M Brook Craven; Diana Radune; Jessica Vamathevan; Hoda Khouri; Owen White; Tanja M Gruber; Karen A Ketchum; J Craig Venter; Hervé Tettelin; Donald A Bryant; Claire M Fraser
Journal:  Proc Natl Acad Sci U S A       Date:  2002-07-01       Impact factor: 11.205

2.  Kinetics of electron transfer between soluble cytochrome c-554 and purified reaction center complex from the green sulfur bacterium Chlorobium tepidum.

Authors:  Masaaki Itoh; Daisuke Seo; Hidehiro Sakurai; Pierre Sétif
Journal:  Photosynth Res       Date:  2002       Impact factor: 3.573

3.  Photo-oxidation of membrane-bound and soluble cytochromec in the green sulfur bacteriumChlorobium tepidum.

Authors:  N Okumura; K Shimada; K Matsuura
Journal:  Photosynth Res       Date:  1994-07       Impact factor: 3.573

4.  Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons.

Authors:  M Karas; F Hillenkamp
Journal:  Anal Chem       Date:  1988-10-15       Impact factor: 6.986

5.  The cytochromes of Chlorobium thiosulfatophilum.

Authors:  T E Meyer; R G Bartsch; M A Cusanovich; J H Mathewson
Journal:  Biochim Biophys Acta       Date:  1968-05-28

6.  Improvement of an "In-Gel" digestion procedure for the micropreparation of internal protein fragments for amino acid sequencing.

Authors:  U Hellman; C Wernstedt; J Góñez; C H Heldin
Journal:  Anal Biochem       Date:  1995-01-01       Impact factor: 3.365

7.  A membrane-bound monoheme cytochrome c551 of a novel type is the immediate electron donor to P840 of the Chlorobium vibrioforme photosynthetic reaction center complex.

Authors:  J S Okkels; B Kjaer; O Hansson; I Svendsen; B L Møller; H V Scheller
Journal:  J Biol Chem       Date:  1992-10-15       Impact factor: 5.157

8.  The amino acid sequences of the cytochromes c-555 from two green sulphur bacteria of the genus Chlorobium.

Authors:  J Van Beeumen; R P Ambler; T E Meyer; M O Kamen; J M Olson; E K Shaw
Journal:  Biochem J       Date:  1976-12-01       Impact factor: 3.857

9.  Characterization of an improved reaction center preparation from the photosynthetic green sulfur bacterium Chlorobium containing the FeS centers FA and FB and a bound cytochrome subunit.

Authors:  U Feiler; W Nitschke; H Michel
Journal:  Biochemistry       Date:  1992-03-10       Impact factor: 3.162

10.  Stable photobleaching of P840 in Chlorobium reaction center preparations: presence of the 42-kDa bacteriochlorophyll a protein and a 17-kDa polypeptide.

Authors:  C Hager-Braun; D L Xie; U Jarosch; E Herold; M Büttner; R Zimmermann; R Deutzmann; G Hauska; N Nelson
Journal:  Biochemistry       Date:  1995-07-25       Impact factor: 3.162
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  1 in total

1.  Isolation and Characterization of an Outer Membrane Protein of Chlorobium tepidum.

Authors:  Michalis Aivaliotis; Elefteria Neofotistou; Hervé-W Rémigy; Georgios Tsimpinos; Ariel Lustig; Friedrich Lottspeich; Georgios Tsiotis
Journal:  Photosynth Res       Date:  2004-02       Impact factor: 3.573
  1 in total

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