AIMS: To study the expression of p63, cytokeratin (CK) 5 and CK8/18 in invasive ductal carcinomas and their relationship with BRCA1 and other pathological and immunohistochemical features of clinical significance. METHODS AND RESULTS: Immunohistochemistry with the antibodies p63, CK5, CK8/18, BRCA1, oestrogen receptor, progesterone receptor, p53, c-erbB-2 and Ki67 was performed in 102 formalin-fixed paraffin-embedded samples of invasive ductal carcinomas. The CK5+ cases were submitted to a double-immunolabelling study with p63. There was a strong relationship between CK5 and p63 expression and both markers were associated with hormonal receptor-negative high-grade carcinomas with high proliferative rate. Furthermore, there was coexpression of CK5 and p63 in neoplastic cells, indicating that p63, like CK5, is a marker of the basal phenotype of breast cancer. There was a strong relationship between reduced expression of BRCA1 with both p63 and CK5 expression as well as an inverse correlation between p63 and CK8/18 expression, suggesting that loss of p63 expression is required for the transition between a basal to a luminal phenotype of breast carcinoma. CONCLUSIONS: Since p63 is thought to be a marker of stem cells and may act as an oncogene, our data support the idea that BRCA1 acts as stem cell regulator.
AIMS: To study the expression of p63, cytokeratin (CK) 5 and CK8/18 in invasive ductal carcinomas and their relationship with BRCA1 and other pathological and immunohistochemical features of clinical significance. METHODS AND RESULTS: Immunohistochemistry with the antibodies p63, CK5, CK8/18, BRCA1, oestrogen receptor, progesterone receptor, p53, c-erbB-2 and Ki67 was performed in 102 formalin-fixed paraffin-embedded samples of invasive ductal carcinomas. The CK5+ cases were submitted to a double-immunolabelling study with p63. There was a strong relationship between CK5 and p63 expression and both markers were associated with hormonal receptor-negative high-grade carcinomas with high proliferative rate. Furthermore, there was coexpression of CK5 and p63 in neoplastic cells, indicating that p63, like CK5, is a marker of the basal phenotype of breast cancer. There was a strong relationship between reduced expression of BRCA1 with both p63 and CK5 expression as well as an inverse correlation between p63 and CK8/18 expression, suggesting that loss of p63 expression is required for the transition between a basal to a luminal phenotype of breast carcinoma. CONCLUSIONS: Since p63 is thought to be a marker of stem cells and may act as an oncogene, our data support the idea that BRCA1 acts as stem cell regulator.
Authors: C Lodillinsky; E Infante; A Guichard; R Chaligné; L Fuhrmann; J Cyrta; M Irondelle; E Lagoutte; S Vacher; H Bonsang-Kitzis; M Glukhova; F Reyal; I Bièche; A Vincent-Salomon; P Chavrier Journal: Oncogene Date: 2015-04-20 Impact factor: 9.867
Authors: Emanuela Heller; Michelle A Hurchla; Jingyu Xiang; Xinming Su; Sara Chen; Jochen Schneider; Kyu-Sang Joeng; Marcos Vidal; Leah Goldberg; Hongju Deng; Mary C Hornick; Julie L Prior; David Piwnica-Worms; Fanxin Long; Ross Cagan; Katherine N Weilbaecher Journal: Cancer Res Date: 2011-12-20 Impact factor: 12.701
Authors: Lamia Benbrahim-Tallaa; Erik J Tokar; Bhalchandra A Diwan; Anna L Dill; Jean-François Coppin; Michael P Waalkes Journal: Environ Health Perspect Date: 2009-08-13 Impact factor: 9.031