Literature DB >> 16237038

Replacement of tyrosine 181 by phenylalanine in gentisate 1,2-dioxygenase I from Pseudomonas alcaligenes NCIMB 9867 enhances catalytic activities.

Chew Ling Tan1, Chew Chieng Yeo, Hoon Eng Khoo, Chit Laa Poh.   

Abstract

xlnE, encoding gentisate 1,2-dioxygenase (EC 1.13.11.4), from Pseudomonas alcaligenes (P25X) was mutagenized by site-directed mutagenesis. The mutant enzyme, Y181F, demonstrated 4-, 3-, 6-, and 16-fold increases in relative activity towards gentisate and 3-fluoro-, 4-methyl-, and 3-methylgentisate, respectively. The specific mutation conferred a 13-fold higher catalytic efficiency (k(cat)/Km) on Y181F towards 3-methylgentisate than that of the wild-type enzyme.

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Year:  2005        PMID： 16237038      PMCID： PMC1272980          DOI： 10.1128/JB.187.21.7543-7545.2005

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

1.  Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

Authors:  R E Parales; K Lee; S M Resnick; H Jiang; D J Lessner; D T Gibson
Journal:  J Bacteriol       Date:  2000-03       Impact factor: 3.490

2.  Site-directed mutagenesis of an extradiol dioxygenase involved in tetralin biodegradation identifies residues important for activity or substrate specificity.

Authors:  Eloísa Andújar; Eduardo Santero
Journal:  Microbiology       Date:  2003-06       Impact factor: 2.777

3.  The enzymic oxidation of gentisic acid.

Authors:  L LACK
Journal:  Biochim Biophys Acta       Date:  1959-07

4.  Gentisate 1,2-dioxygenase from Pseudomonas. Substrate coordination to active site Fe2+ and mechanism of turnover.

Authors:  M R Harpel; J D Lipscomb
Journal:  J Biol Chem       Date:  1990-12-25       Impact factor: 5.157

5.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

6.  Altering catalytic properties of 3-chlorocatechol-oxidizing extradiol dioxygenase from Sphingomonas xenophaga BN6 by random mutagenesis.

Authors:  U Riegert; S Bürger; A Stolz
Journal:  J Bacteriol       Date:  2001-04       Impact factor: 3.490

7.  Evidence for isofunctional enzymes used in m-cresol and 2,5-xylenol degradation via the gentisate pathway in Pseudomonas alcaligenes.

Authors:  C L Poh; R C Bayly
Journal:  J Bacteriol       Date:  1980-07       Impact factor: 3.490

8.  Purification and properties of gentisate 1,2-dioxygenase from Moraxella osloensis.

Authors:  R L Crawford; S W Hutton; P J Chapman
Journal:  J Bacteriol       Date:  1975-03       Impact factor: 3.490

9.  Gentisate 1,2-dioxygenase from pseudomonas. Purification, characterization, and comparison of the enzymes from Pseudomonas testosteroni and Pseudomonas acidovorans.

Authors:  M R Harpel; J D Lipscomb
Journal:  J Biol Chem       Date:  1990-04-15       Impact factor: 5.157

10.  Molecular characterization of an inducible gentisate 1,2-dioxygenase gene, xlnE, from Pseudomonas alcaligenes NCIMB 9867.

Authors:  Chew Chieng Yeo; Mark Vee-Meng Wong; Yongmei Feng; Keang Peng Song; Chit Laa Poh
Journal:  Gene       Date:  2003-07-17       Impact factor: 3.688
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  1 in total

1.  Identification and Characterization of a Novel Gentisate 1,2-Dioxygenase Gene from a Halophilic Martelella Strain.

Authors:  Ling Huang; Haiyang Hu; Hongzhi Tang; Yongdi Liu; Ping Xu; Jie Shi; Kuangfei Lin; Qishi Luo; Changzheng Cui
Journal:  Sci Rep       Date:  2015-09-23       Impact factor: 4.379
  1 in total

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