Novel function of rare catechin, epigallocatechin-3-(3''-O-methyl)gallate, against cold injury in primary rat hepatocytes.

Epigallocatechin-3-(3''-O-methyl)gallate (EGCg-3''-OMe) is a rare component in green tea leaf and its bioactivity is hardly known. In this paper, we report that EGCg-3''-OMe has the function for cold preservation of primary rat hepatocytes. Confluent primary cultured hepatocytes were suspended in a storage solution, culture medium or cell banker (CB). EGCg-3''-OMe was tested as a supplement in the storage solution together with a general cryoprotectant, dimethylsulfoxide (DMSO). After 24 h cold preservation of cells at 4 degrees C followed by 1 h rewarming, cell viability and urea-synthesizing activity, one of the most important liver functions, were measured. EGCg-3''-OMe dose-dependently maintained cell viability and this effect was equal to that of a commercial CB at the highest concentration. Cell viability was also maintained after a further 24 h incubation at 37 degrees C of the cold-preserved hepatocytes. Conversely, urea-synthesizing activity was dose-dependently reduced by EGCg-3''-OMe. Cell protection by EGCg-3''-OMe due to the decrease in metabolic activity in cold-preserved cells was suggested. The decreased hepatic function of cells caused by EGCg-3''-OMe was rescued after a further 24 h incubation of cells at 37 degrees C.