Literature DB >> 16233394

A modified ninhydrin reagent using ascorbic acid instead of potassium cyanide.

Sadaji Yokoyama1, Jun-Ichi Hiramatsu.   

Abstract

The use of a modified ninhydrin reagent using ascorbic acid instead of potassium cyanide was investigated for the photometric determination of amino acids. It has been found possible to obtain an almost stoichiometric reaction for glutamic acid with a quantitative yield of "Ruhemann's purple", the end-product of the reaction, using ascorbic acid as a safe and economical reducing agent. The modified ninhydrin reagent was composed of 0.5 g of ninhydrin, and 15 mg of ascorbic acid in 60 ml of methyl cellosolve.

Entities:  

Year:  2003        PMID： 16233394

Source DB:  PubMed          Journal:  J Biosci Bioeng        ISSN： 1347-4421            Impact factor:   2.894


  7 in total

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2.  Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels.

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6.  Concentration-dependent dual effects of exogenous sucrose on nitrogen metabolism in Andrographis paniculata.

Authors:  Xue-Jing Huang; Shao-Fen Jian; Dong-Liang Chen; Chu Zhong; Jian-Hua Miao
Journal:  Sci Rep       Date:  2022-03-22       Impact factor: 4.996

7.  Carbon and nitrogen metabolic regulation in freshwater plant Ottelia alismoides in response to carbon limitation: A metabolite perspective.

Authors:  Wenmin Huang; Shijuan Han; Liyuan Wang; Wei Li
Journal:  Front Plant Sci       Date:  2022-09-15       Impact factor: 6.627
  7 in total

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